SubtiBank SubtiBank
fliW
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

fliW

checkpoint protein for hag expression, CsrA antagonist
Locus
BSU_35380
Isoelectric point
4.34
Molecular weight
16.03 kDa
Protein length
143 aa Sequence Blast
Gene length
432 bp Sequence Blast
Function
control of CsrA activity
Product
flagellar assembly factor, CsrA antagonist
Essential
no
Synonyms
yviF

Genomic Context

      
Loading

Categories containing this gene/protein

Gene

Coordinates
3,636,264 3,636,695

The protein

Catalyzed reaction/ biological activity

  • FliW binds CsrA when Hag is secreted after hook completion (and FliW released from the Hag-FliW complex) to allow translation of the hag mRNA PubMed
  • Protein family

  • fliW family (single member, according to UniProt)
  • Structure

  • 5DMD (from Geobacillus thermodenitrificans) PubMed
  • 5DMB (FliW-CsrA complex) PubMed
  • Localization

  • cytoplasm (according to Swiss-Prot)
  • Additional information

  • CsrA and FliW form a conserved module in many bacteria PubMed
  • Expression and Regulation

    Operons

    Description

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigD: sigma factor, PubMed, in SigD regulon
  • Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon
  • DegU: activation, DegU-P, PubMed, in DegU regulon
  • ScoC: repression, PubMed, in ScoC regulon
  • view in new tab

    Biological materials

    Mutant

  • MGNA-A339 (yviF::erm), available at the NBRP B. subtilis, Japan
  • BKE35380 (fliW::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTTCACGATCCTTTTC, downstream forward: _UP4_AAGCATCCGATTGGAGGAGA
  • BKK35380 (fliW::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTGTTCACGATCCTTTTC, downstream forward: _UP4_AAGCATCCGATTGGAGGAGA
  • References

    Reviews

    Loading

    Original Publications

    Loading