qdoI [2019-01-02 15:28:42]

Fe-containing quercetin 2,3-dioxygenase

Locus: BSU39980

Isoelectric point: 5.56

Molecular weight: 37.43 kDa

Protein length: 337 aa

Gene length: 1011 bp

Function: resistance to plant product quercetin

Product: Fe-containing quercetin 2,3-dioxygenase

Essential: no

Synonyms: yxaG

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Coping with stress → Resistance against other toxic compounds (nitric oxide, phenolic acids, flavonoids, oxalate)

Gene

Coordinates: 4,106,245 → 4,107,258

The protein

Catalyzed reaction/ biological activity

• oxidation of the flavonol quercetin with dioxygen, cleaving the central heterocyclic ring and releasing CO

Protein family

• bicupin family

Cofactors

• Mn(II) (preferred), but also active with Fe(II) snd Co(II) PubMed

Structure

• 1Y3T PubMed
• 2H0V

Expression and Regulation

Operons

• Genes: qdoI-yxaH
  Description: PubMed

  Regulatory mechanism

  • LmrA: repression, PubMed, in LmrA regulon
  • QdoR: repression, PubMed, in QdoR regulon

  Regulation

  • induced by flavonoids such as quercetin (QdoR, LmrA) PubMed

  Additional information

  • major regulator: QdoR PubMed

Biological materials

Mutant

• MGNA-B684 (yxaG::erm), available at the NBRP B. subtilis, Japan
• BKE39980 (ΔqdoI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTATCGCTTCCTCCAA, downstream forward: _UP4_TAAAGATGACAAGATTGTAA
• BKK39980 (ΔqdoI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATTATCGCTTCCTCCAA, downstream forward: _UP4_TAAAGATGACAAGATTGTAA

References

Reviews

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Original publications

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