yisV

similar to transcriptional regulator (MocR/ GabR family)

Locus: BSU_10880

Isoelectric point: 9.30

Molecular weight: 54.11 kDa

Protein length: 484 aa

Gene length: 1455 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: yisW

Genomic Context

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Categories containing this gene/protein

• Information processing → Regulation of gene expression → Transcription factors and their control → Transcription factor/ other/ based on similarity

Gene

Coordinates: 1,166,737 1,168,191

The protein

Protein family

• MocR/ GabR family PubMed
• class-I pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Paralogous protein(s)

• YdfD

Domains

• N-terminal DNA-binding helix-turn-helix motif; C-terminal domain is homologous to PLP-binding large domain of aminotransferases.
• HTH gntR-type domain (aa 14-82) (according to UniProt)

Cofactors

• PLP

Structure

• 1WST (aminotransferase from Thermococcus profundus, 29% identity)

Expression and Regulation

Operons

• Genes: yisV
  Description: PubMed

Biological materials

Mutant

• MGNA-B211 (yisW::erm), available at the NBRP B. subtilis, Japan
• BKE10880 (yisV::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCCCCCCTTTTTTACAC, downstream forward: _UP4_CGCCTGATGATGTCCTAGGC
• BKK10880 (yisV::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCCCCCCTTTTTTACAC, downstream forward: _UP4_CGCCTGATGATGTCCTAGGC

References

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