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mreC [Tue Dec 08 2015 12:51:41 GMT+0100 (CET)]
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mreC [Tue Dec 08 2015 12:51:41 GMT+0100 (CET)]

MreC is a cell shape determining protein, it couples the cytosolic MreB and MreB-like proteins to the extracellular peptidoglycan-synthesizing machinery
locus
BSU28020
pI
6.00
mw
32.00 kDa
function
cell shape determation
product
cell shape-determining protein
essential
yes
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
  • [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|cell shape], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)], [SW|essential genes], [SW|membrane proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|SigM regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,859,832 -> 2,860,704

    Phenotypes of a mutant

  • ''mreC'' is essential under normal conditions [Pubmed|12682299].
  • Depletion of MreC leads to a progressive increase in the width and a decrease in the length of the cell. This shape defect is consistent with a role for ''mreC'' in cell wall synthesis during elongation and has a similar phenotype to other genes with roles in elongation like ''[protein|search|rodA]'' and the redundant gene pair ''[protein|search|pbpA]'' and ''[protein|search|pbpH]''.
  • Electron microscopy of cells depleted of MreC shows regions of the cell where a thick and irregular cell wall has accumulated [Pubmed|12867458,16101995].
  • ''mreC'' can be deleted provided that 0.5 M sucrose and 20 mM Mg(2 ) is provided in the media, ''mreC'' is therefore conditionally essential. The phenotype of the ''mreC'' deletion in these conditions is one characterised by extreamly fat and bloated cells that tend to grow in clusters [Pubmed|16101995].

    • The protein

    Catalyzed reaction/ biological activity

  • None/ structural protein

    • Protein family

  • mreC family (according to Swiss-Prot) COG1793

    • Paralogous protein(s)

  • None

    • [SW|Domains]

  • Intracellular N-terminus, transmembrane domain, Coiled coil domain and C-terminal beta-sheet domain.

    • Structure

  • [PDB|2J5U]: MreC from ''Listeria monocytogenes'' [Pubmed|17427287]
  • [PDB|2QF4]: MreC monomer from ''Streptococcus pneumoniae'' [Pubmed|17707860]
  • [PDB|2QF5]: MreC dimer from ''Streptococcus pneumoniae'' [Pubmed|17707860]

    • [SW|Localization]

  • trans-membrane protein [Pubmed|21636744,16101995]
  • during logarithmic growth, [protein|search|MreD] forms discrete patches thst move processively along peripheral tracks perpendicular to the cell axis [Pubmed|21636744]
  • forms transverse bands as cells enter the stationary phase [Pubmed|21636744]
  • reports on helical structures formed by MreC [Pubmed|16101995,20566861] seem to be misinterpretation of data [Pubmed|21636744]

    • [SW|Interactions]

  • part of the [SW|cell wall biosynthetic complex] [Pubmed|21636744,21636745]
  • [protein|search|MreC]-[protein|search|MreD], interacts also with a subset of the [penicillin-binding protein|search|PBPs] [Pubmed|17427287]
  • [protein|search|MreC]-[protein|search|GpsB] [Pubmed|18363795]

    • Expression and Regulation

    Operon

  • ''[protein|search|radC]-[protein|search|mreB]-[protein|search|mreC]-[protein|search|mreD]-[protein|search|minC]-[protein|search|minD]'' [Pubmed|18179421]
  • ''[protein|search|mreB]-[protein|search|mreC]-[protein|search|mreD]-[protein|search|minC]-[protein|search|minD]'' [Pubmed|8459776]

    • [SW|Sigma factor]

  • ''[protein|search|radC]'': [protein|search|SigM] [Pubmed|18179421]

    • Regulation

  • expression is reduced in a [protein|search|SigV] mutant [Pubmed|21926231]

    • Biological materials

    Mutant

  • A non-polar inframe deletion strain named 3481 and a xylose dependent conditional mutant named 3461 is avaliable from the [SW|Errington] lab [Pubmed|16101995].

    • GFP fusion

  • A functional N-terminal GFP fusion has been made where the fusion protein is the only copy of the gene in the cell: strain 3417 [Pubmed|16101995].

    • Antibody

  • antisera raised in rabit is avaliable from the [SW|Errington] lab.

    • Labs working on this gene/protein

  • [SW|Jeff Errington], Newcastle University, UK [http://www.ncl.ac.uk/camb/staff/profile/jeff.errington homepage]
  • [SW|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]

    • References

  • 20566861,21636744,21636745,18363795,17427287,18179421,8459776,21926231,16101995