ytrA

transcriptional repressor, [SW|GntR family], control of cell envelope stress responses in response to ramoplanin

locus: BSU_30460

pI: 8.09

mw: 14.55 kDa

protein length: 130 aa

gene length: 393 bp

function: control of cell envelope stress responses

product: transcriptional repressor ([SW|GntR family])

essential: no

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.9|Newly identified competence genes]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.3|Sporulation/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.5|Cold stress proteins]

Gene

Coordinates: 3,118,847 3,119,239

Phenotypes of a mutant

• non-transformable [pubmed|28189581]
• reduced sporulation efficiency [pubmed|28189581]

The protein

Protein family

• [SW|GntR family] of transcription factors

[SW|Domains]

• [SW|HTH gntR-type domain] (aa 10-78) (according to UniProt)

Structure

• [PDB|3NEU] (similar protein from ''Listeria innocua'', 41% identity)

Expression and Regulation

Operons

• genes: [gene|DCFA6025F7B4D7C5F6FB8107DDA6538CED33084D|ytrG]-[gene|6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|ytrA]-[gene|BF81AD95FE6CF7662D012EF293C769E80C093D34|ytrB]-[gene|31977FDC549E45911C333623DCA3D8941A93D8FF|ytrC]-[gene|9C8233C48733A1E9271EE7AA1283EA9F446CF9DA|ytrD]-[gene|4FE6A51A0D9BBBF574EA2EF18B95B5BFC0A64CCB|ytrE]-[gene|9024AB63030C1934A954EE6A378CADBDC977E863|ytrF]
  description: [Pubmed|10986249,21856850]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10986249,21856850], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|YtrA]: repression, [Pubmed|10986249,21856850], in [regulon|6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|YtrA regulon]

  regulation

  • expressed early in the stationary phase [Pubmed|10986249]

Biological materials

Mutant

• GP2641 (Δ[gene|6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|ytrA]::''spc''), available in [SW|Jörg Stülke]'s lab
• GP2647 (Δ[gene|6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|ytrA]::''ermC''), available in [SW|Jörg Stülke]'s lab
• GP2646 Δ([gene|DCFA6025F7B4D7C5F6FB8107DDA6538CED33084D|ytrG]-[gene|6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|ytrA]-[gene|BF81AD95FE6CF7662D012EF293C769E80C093D34|ytrB]-[gene|31977FDC549E45911C333623DCA3D8941A93D8FF|ytrC]-[gene|9C8233C48733A1E9271EE7AA1283EA9F446CF9DA|ytrD]-[gene|4FE6A51A0D9BBBF574EA2EF18B95B5BFC0A64CCB|ytrE]-[gene|9024AB63030C1934A954EE6A378CADBDC977E863|ytrF])::''ermC'', available in [SW|Jörg Stülke]'s lab
• MGNA-A138 (ytrA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/138 NBRP B. subtilis, Japan]
• BKE30460 ([gene|6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|ytrA]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE30460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTACTTCCCTACTTTCT, downstream forward: _UP4_GCTGATGTGAAGGGAGGCAA
• BKK30460 ([gene|6A074CE9AD98A2751A216A2EA8156ACE6FFC7280|ytrA]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK30460 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTACTTCCCTACTTTCT, downstream forward: _UP4_GCTGATGTGAAGGGAGGCAA

References

• 10986249,12399512,23504016,21856850,28189581