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yjbQ [2018-09-26 12:13:10]
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yjbQ [2018-09-26 12:13:10]

similar to Na /H antiporter
Locus
BSU11640
Isoelectric point
5.25
Molecular weight
67.29 kDa
Protein length
614 aa Sequence Blast
Gene length
1842 bp Sequence Blast
Function
unknown
Product
unknown
Essential
no
Synonyms
cpaA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Cellular processes → Homeostasis → Metal ion homeostasis (K, Na, Ca, Mg) → Potassium uptake/ export
  • Information processing → Targets of second messengers → Targets of c-di-AMP
  • Groups of genes → Membrane proteins

    • Gene

    Coordinates
    1,240,356 → 1,242,200

    The protein

    Domains

  • contains a RCK_N domain (according to UniProt)
  • contains a RCK_C domain at the C-terminus (according to UniProt)

    • Localization

  • cell membrane (according to Swiss-Prot)

    • Expression and Regulation

    Operons

    Genes
    cpaA
    Description
    PubMed
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    Biological materials

    Mutant

  • MGNA-B163 (yjbQ::erm), available at the NBRP B. subtilis, Japan
  • GP2082 (cpaA::ermC), available in Jörg Stülke's lab
  • BKE11640 (ΔcpaA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATCAGAACCTCCTTTT, downstream forward: _UP4_TAAATCTTGACCAAATAAGG
  • BKK11640 (ΔcpaA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATCAGAACCTCCTTTT, downstream forward: _UP4_TAAATCTTGACCAAATAAGG

    • Expression vector

  • pGP2909 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab

    • FLAG-tag construct

  • GP2438 yjbQ-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab

    • References

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