yjbQ [2018-11-25 17:47:31]

similar to Na⁺/H⁺ antiporter

Locus: BSU11640

Isoelectric point: 5.25

Molecular weight: 67.29 kDa

Protein length: 614 aa

Gene length: 1842 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: cpaA

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Homeostasis → Metal ion homeostasis (K, Na, Ca, Mg) → Potassium uptake/ export
• Information processing → Targets of second messengers → Targets of c-di-AMP
• Groups of genes → Membrane proteins

Gene

Coordinates: 1,240,356 → 1,242,200

The protein

Domains

• contains a RCK_N domain (according to UniProt)
• contains a RCK_C domain at the C-terminus (according to UniProt)

Structure

• 4BWZ (from Thermus thermophilus, corresponds to the transmembrane domain (aa 27 ... 402), 26% identity) PubMed

Localization

• cell membrane (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: cpaA
  Description: PubMed

Biological materials

Mutant

• MGNA-B163 (yjbQ::erm), available at the NBRP B. subtilis, Japan
• GP2082 (cpaA::ermC), available in Jörg Stülke's lab
• BKE11640 (ΔcpaA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATCAGAACCTCCTTTT, downstream forward: _UP4_TAAATCTTGACCAAATAAGG
• BKK11640 (ΔcpaA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAATCAGAACCTCCTTTT, downstream forward: _UP4_TAAATCTTGACCAAATAAGG

Expression vectors

• pGP2909 (N-terminal His-tag, purification from E. coli, in pWH844), available in Jörg Stülke's lab

FLAG-tag construct

• GP2438 yjbQ-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab

References

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