rocA [Mon May 16 2016 17:49:43 GMT+0200 (CEST)]

3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase

locus: BSU37780

pI: 5.00

mw: 56.00 kDa

function: arginine, ornithine and citrulline utilization

product: 3-hydroxy-1-pyrroline-5-carboxylate dehydrogenase

essential: no

ec: 1.5.1.12

synonyms: ipa-76d

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.4|Utilization of arginine/ ornithine]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.8|Phosphorylation on either a Ser, Thr or Tyr residue]

[SW|Categories] containing this gene/protein

• [SW|utilization of amino acids], [SW|phosphoproteins]

This gene is a member of the following [SW|regulons]

• [SW|AbrB regulon], [SW|AhrC regulon], [SW|CodY regulon], [SW|RocR regulon], [SW|SigL regulon]

Gene

Coordinates on the chromosome (coding sequence): 3,878,966 -> 3,880,513

The protein

Catalyzed reaction/ biological activity

• (S)-1-pyrroline-5-carboxylate NAD(P) 2 H₂O = L-glutamate NAD(P)H (according to Swiss-Prot)

Protein family

• RocA subfamily (according to Swiss-Prot)

Paralogous protein(s)

• [protein|search|PutC], additional similar proteins: [protein|search|YfmT], [protein|search|AldY], [protein|search|YcbD], [protein|search|GabD], [protein|search|GbsA], [protein|search|DhaS], [protein|search|IolA], [protein|search|AldX], [protein|search|YwdH]

Modification

• phosphorylation on (Thr-2 OR Thr-4 OR Tyr-5) [Pubmed|17218307]

Structure

• [PDB|3RJL] (1-pyrroline-5-carboxylate dehydrogenase from ''Bacillus licheniformis'', 68% identity, 88% similarity)

Expression and Regulation

Operon

• ''[protein|search|rocA]-[protein|search|rocB]-[protein|search|rocC]'' [Pubmed|8113162]

[SW|Sigma factor]

• [protein|search|SigL] [Pubmed|8113162]

Regulation

• induction by arginine ([protein|search|RocR], [protein|search|AhrC]) [http://www.ncbi.nlm.nih.gov/sites/entrez/8113162,7565595 PubMed]
• repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]

Regulatory mechanism

• [protein|search|CodY]: transcription repression [Pubmed|12618455]
• [protein|search|RocR]: transcription activation [Pubmed|8113162]
• [protein|search|AhrC]: transcription activation [Pubmed|7565595]
• [protein|search|AbrB]: transcription repression [Pubmed|20817675]

References

• 7565595,12618455,8113162,17218307,20817675