csoR

transcription repressor of [gene|18506FB74F6BB9278F22EB7DCDA3CF72575CC32A|copZ]-[gene|727024F7B1AC19676ED4B516CF46A47B1328310B|copA]

locus: BSU_33520

pI: 7.30

mw: 11.34 kDa

protein length: 101 aa

gene length: 306 bp

function: control of copper homeostasis

product: transcription repressor

essential: no

synonyms: yvgZ

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.3|Homeostasis] → [category|SW 1.3.2|Trace metal homeostasis (Cu, Zn, Ni, Mn, Mo)] → [category|SW 1.3.2.1|Copper]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.11|Resistance against toxic metals]
• [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.1|Phosphorylation on an Arg residue]

Gene

Coordinates: 3,443,896 3,444,201

The protein

Protein family

• CsoR family (single member, according to UniProt)

Effectors of protein activity

• Cu(I) ions cause release of CsoR from its operator sites

Structure

• [PDB|4M1P] (from Geobacillus thermodenitrificans, 59% identity) [pubmed|24831014]

Expression and Regulation

Operons

• genes: [gene|6977F18870004AD236539D9409255815E6BE9241|csoR]-[gene|18506FB74F6BB9278F22EB7DCDA3CF72575CC32A|copZ]-[gene|727024F7B1AC19676ED4B516CF46A47B1328310B|copA]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B604 (yvgZ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1603 NBRP B. subtilis, Japan]
• BKE33520 ([gene|6977F18870004AD236539D9409255815E6BE9241|csoR]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE33520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCGTACACCTCTGTTTA, downstream forward: _UP4_TAAAGCGTTTTTTATTGTAA
• BKK33520 ([gene|6977F18870004AD236539D9409255815E6BE9241|csoR]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK33520 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGCGTACACCTCTGTTTA, downstream forward: _UP4_TAAAGCGTTTTTTATTGTAA

labs

• [SW|John Helmann], Cornell University, USA [http://www.micro.cornell.edu/research/labs/helmann-lab/index.cfm Homepage]
• [SW|Mohamed Marahiel], Marburg University, Germany [http://www.uni-marburg.de/fb15/fachgebiete/bio/marahiel?language_sync=1 homepage]

References

Reviews

• 22918892,25209494

Original publications

• 18048925,19168619,20233928,19168619,19249860,24831014