yojI

similar to multidrug efflux transporters

locus: BSU_19440

pI: 9.78

mw: 49.09 kDa

protein length: 452 aa

gene length: 1359 bp

function: unknown

product: putative multidrug exporter

essential: no

synonyms: norM

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.4|Transporters/ other] → [category|SW 1.2.4.15|Multidrug exporters/ based on homology]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.14|Resistance against toxins/ antibiotics/ based on similarity]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,119,393 2,120,751

The protein

Protein family

• [SW|MOP exporter family]
• [SW|multi antimicrobial extrusion (MATE) (TC 2.A.66.1) family] (according to UniProt)

Structure

• [PDB|3MKT] (multidrug exporter from Vibrio cholerae, 37% identity) [pubmed|20861838]

[SW|Localization]

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|692C2F92DBE9E917697A883DA66C6E0FA2DE3FBA|yojI]-[gene|5EEEDE60CE9E5CDAEBAB0E03AABDF1DF62F1F002|rsbRC]
  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B427 (yojI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1426 NBRP B. subtilis, Japan]
• BKE19440 ([gene|692C2F92DBE9E917697A883DA66C6E0FA2DE3FBA|yojI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE19440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACATTGATCTCCGTTTC, downstream forward: _UP4_CACATTTAAAAGAGGTGATC
• BKK19440 ([gene|692C2F92DBE9E917697A883DA66C6E0FA2DE3FBA|yojI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK19440 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATACATTGATCTCCGTTTC, downstream forward: _UP4_CACATTTAAAAGAGGTGATC

References

Research papers

• 20861838