ytzG

similar to 16S pseudouridylate synthase

Locus: BSU_30035

Isoelectric point: 5.62

Molecular weight: 27.00 kDa

Protein length: 239 aa

Gene length: 720 bp

Function: 16S rRNA modification

Product: putative 16S pseudouridylate synthase

Essential: no

E.C.: 5.4.99.19

Synonyms

Genomic Context

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Categories containing this gene/protein

• Information processing → Protein synthesis, modification and degradation → Translation → rRNA modification and maturation/ based on similarity

Gene

Coordinates: 3,072,743 3,073,462

The protein

Catalyzed reaction/ biological activity

• uridine⁵¹⁶ in 16S rRNA --> a pseudouridine⁵¹⁶ in 16S rRNA (according to E. coli RsuA)

Protein family

• S4 RNA-binding domain superfamily (according to http://www.ebi.ac.uk/interpro/entry/IPR036986 Interpro)
• pseudouridine synthase RsuA family (with RluB, according to UniProt)

Domains

• S4 RNA-binding domain (aa 1-65) (according to UniProt)

Structure

• 1KSK (RsuA from E. coli, 40% identical) PubMed

Expression and Regulation

Operons

• Genes: murJ-ytzG
  Description: PubMed

Biological materials

Mutant

• BKE30035 (ytzG::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCCAATCTCTCCTTTA, downstream forward: _UP4_TGACAAAAACCCCGCATACC
• BKK30035 (ytzG::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTCCCAATCTCTCCTTTA, downstream forward: _UP4_TGACAAAAACCCCGCATACC

References

Research papers

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