bioA

lysine-8-amino-7-oxononanoate aminotransferase

Locus: BSU_30230

Isoelectric point: 5.32

Molecular weight: 49.95 kDa

Protein length: 448 aa

Gene length: 1344 bp

Function: biosynthesis of biotin

Product: lysine-8-amino-7-oxononanoate aminotransferase

Essential: no

E.C.: 2.6.1.62

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of biotin

Gene

Coordinates: 3,093,343 → 3,094,689

The protein

Catalyzed reaction/ biological activity

• 8-amino-7-oxononanoate + L-lysine --> (S)-2-amino-6-oxohexanoate + 7,8-diaminononanoate (according to UniProt)

Protein family

• Class-III pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Paralogous protein(s)

• YhxA:

Cofactors

• PLP (according to UniProt)

Structure

• 3DRD

Expression and Regulation

Operons

• Genes: bioW-bioA-bioF-bioD-bioB-bioI-ytbQ
  Description: PubMed

  Regulatory mechanism

  • BirA: repression, PubMed, in BirA regulon

  Regulation

  • repressed in the presence of biotin (BirA) PubMed

Biological materials

Mutant

• BKE30230 (ΔbioA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATCATGAGTCATGATCTTC, downstream forward: _UP4_AGCCTTGAAGATTGATTCCT
• BKK30230 (ΔbioA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_AATCATGAGTCATGATCTTC, downstream forward: _UP4_AGCCTTGAAGATTGATTCCT

References

Reviews

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Original publications

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