malR

two-component response regulator, regulation of malate uptake

Locus: BSU_31530

Isoelectric point: 5.98

Molecular weight: 26.82 kDa

Protein length: 235 aa

Gene length: 708 bp

Function: regulation of malate uptake

Product: two-component response regulator

Essential: no

Synonyms: yufM

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of organic acids
• Information processing → Regulation of gene expression → Transcription factors and their control → Two-component system response regulators
• Groups of genes → Phosphoproteins → Phosphorylation on an Asp residue

Gene

Coordinates: 3,238,751 3,239,458

Phenotypes of a mutant

• unable to use malate as the single carbon source PubMed

The protein

Domains

• Response regulatory domain (aa 3-119) (according to UniProt)

Modification

• phosphorylated by MalK on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Structure

• 5LWK (from Lactobacillus paracasei, N-terminal part, corresponds to aa 1 ... 122, 43% identity) PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: malR
  Description: PubMed

• Genes: malK-malR
  Description: PubMed

Biological materials

Mutant

• MGNA-A635 (yufM::erm), available at the NBRP B. subtilis, Japan
• GP1301 (cat), available in Jörg Stülke's lab
• BKE31530 (malR::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTAATCATGATTTTCCTC, downstream forward: _UP4_TAAGAAAACGCACTGCTTGC
• BKK31530 (malR::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTAATCATGATTTTCCTC, downstream forward: _UP4_TAAGAAAACGCACTGCTTGC

Labs working on this gene/protein

• Stephane Aymerich, Microbiology and Molecular Genetics, INRA Paris-Grignon, France

References

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