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pucE
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pucE

xanthine dehydrogenase
locus
BSU_32470
pI
6.13
mw
18.69 kDa
protein length
173 aa Sequence Blast
gene length
522 bp Sequence Blast
function
purine utilization
product
xanthine dehydrogenase
essential
no
synonyms
yurB
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.5|Nucleotide metabolism] → [category|SW 2.5.1|Utilization of nucleotides]

    • Gene

    Coordinates
    3,335,751 3,336,272

    The protein

    Catalyzed reaction/ biological activity

  • H2O + NAD+ + xanthine --> H+ + NADH + urate (according to UniProt)
  • H2O + hypoxanthine + NAD+ --> H+ + NADH + xanthine (according to UniProt)

    • [SW|Domains]

  • 2Fe-2S ferredoxin-type domain (aa 14-90) (according to UniProt)

    • [SW|Cofactors]

  • Fe-S cluster [pubmed|29292548]

    • Structure

  • [PDB|1JRO] (from ''Rhodobacter capsulatus'', 43% identity, 58% similarity) [Pubmed|11796116]

    • Expression and Regulation

    Operons

    genes
    [gene|C9370B221117B89A1134B413748D39505C2E1DA8|pucA]-[gene|99428BD0B1A8E91F4B028ACF8AB51B235DDB4F11|pucB]-[gene|D0F00ACF5AAD9E9418033F5B47527C4D50007F18|pucC]-[gene|B65E4B340961332ED0ACC7FD957351256F708F60|pucD]-[gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]
    description
    [Pubmed|12823818]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|12029039], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|52C1601482C26400A524E880334BB801F832D6ED|PucR]: activation, [Pubmed|12029039], in [regulon|52C1601482C26400A524E880334BB801F832D6ED|PucR regulon]

    • regulation

  • induced in the presence of purine nucleotides (effector: allantoin) ([protein|search|PucR]) [Pubmed|12029039]
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    Biological materials

    Mutant

  • MGNA-A939 (yurB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/939 NBRP B. subtilis, Japan]
  • BKE32470 ([gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE32470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACAGGAAATGGCCCGGCCT, downstream forward: _UP4_TAAAAGCCGGACTTTTTTAG
  • BKK32470 ([gene|6855CC156FCB4C4A16AFD6ADE18F3FFC82B61843|pucE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK32470 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TACAGGAAATGGCCCGGCCT, downstream forward: _UP4_TAAAAGCCGGACTTTTTTAG

    • References

  • 11344136,12029039,12823818