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sacT [Tue Jul 05 2016 18:05:24 GMT+0200 (CEST)]

transcriptional antiterminator for the sacP-sacA-ywdA operon

locus

BSU38070

5.00

31.00 kDa

function

regulation of sucrose utilization

product

transcriptional antiterminator

essential

synonyms

ipa-47d

Outlinks

KEGG BsubCyc SubtiList UniProt Expression Browser

Genomic Context

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categories

[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.19|Utilization of sucrose]

[category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.3|PRD-type regulators]

[category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.4|RNA binding regulators]

[category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.9|Phosphoproteins / Other]

[SW|Categories] containing this gene/protein

[SW|utilization of specific carbon sources], [SW|transcription factors and their control], [SW|RNA binding regulators], [SW|phosphoproteins]

This gene is a member of the following [SW|regulons]

[SW|DnaA regulon]

Gene

Coordinates on the chromosome (coding sequence)

3,906,142 -> 3,906,972

The protein

Catalyzed reaction/ biological activity

binding to the mRNA of the ''[protein|search|sacP]-[protein|search|sacA]'' operon, causes transcription antitermination (in presence of sucrose and absence of glucose)

Protein family

[PRD-containing transcription factors|search|transcriptional antiterminator] bglG family (according to Swiss-Prot) BglG family of antiterminators

Paralogous protein(s)

[SW|Interactions]

[protein|search|PtsH]-[protein|search|SacT]

Expression and Regulation

Operon

''[protein|search|sacT]'' [Pubmed|9353933]

Regulation

repressed by casamino acids [Pubmed|12107147]

Regulatory mechanism

[protein|search|DnaA]: transcription repression [Pubmed|26020636]

Biological materials

Mutant

GP429 (spc), available in [SW|Jörg Stülke]'s lab

Expression vector

for expression, purification of both PRDs in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP166, available in [SW|Jörg Stülke]'s lab

for expression, purification of PRD-1 in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP426, available in [SW|Jörg Stülke]'s lab

for expression, purification of PRD-2 in ''E. coli'' with N-terminal His-tag, in [SW|pWH844]: pGP427, available in [SW|Jörg Stülke]'s lab

for expression, purification of PRD-1 in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [SW|pGP570]: pGP439, available in [SW|Jörg Stülke]'s lab

for expression, purification of PRD-2 in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [SW|pGP570]: pGP440, available in [SW|Jörg Stülke]'s lab

for expression, purification of the RNA-binding domain in ''E. coli'' with N-terminal His-tag and thrombin cleavage site, in [SW|pGP570]: pGP571, available in [SW|Jörg Stülke]'s lab

for expression of the RNA-binding domain in ''B. subtilis'', in [SW|pBQ200]: pGP446, available in [SW|Jörg Stülke]'s lab

for expression, purification of [protein|search|sacT]-full length in ''B. subtilis'' with C-terminal Strep-tag, in [SW|pGP382]: pGP1064, available in [SW|Jörg Stülke]'s lab

for expression, purification of [protein|search|sacT]-full length in ''B. subtilis'' with N-terminal Strep-tag, in [SW|pGP380]: pGP1068, available in [SW|Jörg Stülke]'s lab

GFP fusion

GP1227 (spc, based on [SW|pGP1870]), available in [SW|Jörg Stülke]'s lab

FLAG-tag construct

GP1223 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab

References

12107147,2163394,1577686,8702561,1279678,21278164,26020636,22383849