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ssuB [2018-05-04 14:19:43]
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ssuB [2018-05-04 14:19:43]

aliphatic sulfonate ABC transporter (binding protein)
locus
BSU08830
pI
8.67
mw
30.08 kDa
protein length
270 aa Sequence Blast
gene length
810 bp Sequence Blast
function
sulfonate uptake
product
aliphatic sulfonate ABC transporter (binding protein)
essential
no
synonyms
ygaL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.6|Uptake of ions]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.2|sulfur metabolism/ general]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    961,394 → 962,161

    The protein

    Protein family

  • [SW|ABC transporter] domain (according to Swiss-Prot)

    • [SW|Localization]

  • associated to the membrane (via [protein|91EAC386AD3A373E7CDE0265AA23786ADC55558A|SsuC]) [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|675CE0BCA393E9C2795032F1C0FDE6AF08B77033|ssuB]-[gene|B47C64731FCAA086CA563EEE95D3205428E51EFC|ssuA]-[gene|91EAC386AD3A373E7CDE0265AA23786ADC55558A|ssuC]-[gene|A9F7BEC330EB17CA38C4D421E8BBF58CE380559C|ssuD]-[gene|93F7A5795A423CD8DD7B070F7F37731A5E8D6321|ygaN]
    description
    [Pubmed|11251850]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11251850], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]

    • regulation

  • repressed in the presence of cysteine ([protein|search|CymR]) [Pubmed|16513748]
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    Biological materials

    Mutant

  • MGNA-A249 (ygaL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/249 NBRP B. subtilis, Japan]
  • BKE08830 (Δ[gene|675CE0BCA393E9C2795032F1C0FDE6AF08B77033|ssuB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTATCCCTCTCTTTC, downstream forward: _UP4_ATATAGAAAGGGCGGAAGCG
  • BKK08830 (Δ[gene|675CE0BCA393E9C2795032F1C0FDE6AF08B77033|ssuB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTATCCCTCTCTTTC, downstream forward: _UP4_ATATAGAAAGGGCGGAAGCG

    • References

  • 15937167,11251850,10092453,16513748,9782504