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ssuB [2018-05-04 14:23:40]
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ssuB [2018-05-04 14:23:40]

aliphatic sulfonate [SW|ABC transporter] (binding protein)
locus
BSU08830
pI
8.67
mw
30.08 kDa
protein length
270 aa Sequence Blast
gene length
810 bp Sequence Blast
function
sulfonate uptake
product
aliphatic sulfonate [SW|ABC transporter] (binding protein)
essential
no
synonyms
ygaL
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.1|Importers] → [category|SW 1.2.1.1.6|Uptake of ions]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.4|Sulfur metabolism] → [category|SW 2.6.4.2|sulfur metabolism/ general]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    961,394 → 962,161

    The protein

    Protein family

  • [SW|ABC transporter] domain (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|3DC6A032B0F076A460FE2C3244D747CFC7583FE7|YtlC]

    • Structure

  • [PDB|1Q1B] (MalK from E. coli, 38% identity)

    • [SW|Localization]

  • associated to the membrane (via [protein|91EAC386AD3A373E7CDE0265AA23786ADC55558A|SsuC]) [Pubmed|10092453]

    • Expression and Regulation

    Operons

    genes
    [gene|675CE0BCA393E9C2795032F1C0FDE6AF08B77033|ssuB]-[gene|B47C64731FCAA086CA563EEE95D3205428E51EFC|ssuA]-[gene|91EAC386AD3A373E7CDE0265AA23786ADC55558A|ssuC]-[gene|A9F7BEC330EB17CA38C4D421E8BBF58CE380559C|ssuD]-[gene|93F7A5795A423CD8DD7B070F7F37731A5E8D6321|ygaN]
    description
    [Pubmed|11251850]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|11251850], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|CymR]: repression, [Pubmed|16513748], in [regulon|50930C56C27D22715620A350220E3C56ADB41020|CymR regulon]

    • regulation

  • repressed in the presence of cysteine ([protein|search|CymR]) [Pubmed|16513748]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-A249 (ygaL::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/249 NBRP B. subtilis, Japan]
  • BKE08830 (Δ[gene|675CE0BCA393E9C2795032F1C0FDE6AF08B77033|ssuB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE08830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTATCCCTCTCTTTC, downstream forward: _UP4_ATATAGAAAGGGCGGAAGCG
  • BKK08830 (Δ[gene|675CE0BCA393E9C2795032F1C0FDE6AF08B77033|ssuB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK08830 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTTTATCCCTCTCTTTC, downstream forward: _UP4_ATATAGAAAGGGCGGAAGCG

    • References

  • 15937167,11251850,10092453,16513748,9782504