dnaA [2019-01-03 14:03:00]

AAA ATPase, [category|SW 3.1.1|replication] initiation protein

locus: BSU00010

pI: 6.03

mw: 50.70 kDa

protein length: 446 aa

gene length: 1338 bp

function: [category|SW 3.1.1|DNA replication]

product: replication initiation protein

essential: yes

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication]
• [category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes]

Gene

Coordinates: 410 → 1,750

Phenotypes of a mutant

• essential [Pubmed|12682299]

The protein

Catalyzed reaction/ biological activity

• binds multiple regions in the oriC region, required for recruitment of proteins needed to load the replicative helicase [protein|5CF09E089AA70A32F640CE0D33D1F05312AAE24D|DnaC]

Protein family

• dnaA family (according to Swiss-Prot)

[SW|Domains]

• AAA + domain

Effectors of protein activity

• [protein|03595BF6DB8AE79604A720A5BD85C0DB781EE306|SirA] displaces [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] from the replication origin [Pubmed|19682252]
• [protein|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|YabA] inhibits co-operative binding of DnaA to the ''oriC'' DNA [Pubmed|21895792]
• DnaA helix formation (and thus replication initiation) is inhibited by the interaction of either [protein|5B3DB5A796ACA48390278E60478DFF13D0074A8D|ParA], [protein|A039228A3805F4E7C5C2AE31C7DB0808562E88E3|YabA] or [protein|83E05071DEC874248D3E96B8F3A093C65939B314|DnaN] with the AAA domain of DnaA [Pubmed|23909787,22286949]
• interaction with [protein|C9FEE07601AD28B2651EB881A1D9B34D5082237C|DnaD] inhibits the ability of [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] to cooperatively bind to DNA [Pubmed|22821970]
• DNA gyrase ([protein|B207C7328177924B16F1C606F667BEADF12CA069|GyrA]-[protein|AA42910B71348EFEA4165CFFCEF1E497897B9DDC|GyrB]) inhibits association of DnaA with [gene|search|oriC] [pubmed|29396913]

Structure

• [PDB|2Z4R] (from ''Thermotoga maritima '') [Pubmed|18216012]

[SW|Localization]

• throughout the cytoplasm [Pubmed|10844689]
• co-localizes with the origin region and replication forks throughout the cell cycle [pubmed|28166228]

Expression and Regulation

Operons

• genes: [gene|6740108089F13116F200C15F35C2E7561E990FEB|dnaA]-[gene|83E05071DEC874248D3E96B8F3A093C65939B314|dnaN]
  description: [Pubmed|2987848]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|2987848], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A]: repression, [Pubmed|14651647], in [regulon|2C54FE2ADC82FF414D732018C90649D477A925AD|Spo0A regulon]

  regulation

  • negatively controlled by [protein|search|DnaA] [Pubmed|2168872] and [SW|Spo0A] [Pubmed|14651647]

  additional information

  • term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''dnaA'' [Pubmed|27120414]

labs

• [SW|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage]
• [SW|Alan Grossman], MIT, Cambridge, MA, USA
• [SW|Heath Murray], Centre for Bacterial Cell Biology, Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/heath.murray homepage]

References

Reviews

• 20157337,21035377,21639790,22575476,22797751,26706151,28075389

The [SW|DnaA regulon]

• 16120674

Original publications

• 20511500,21097613,21239581,21895792,22286949,22821970,23909787,22396664,21911367,25041308,25340815,26020636,18854156,19011033,11222620,14651647,17140409,10844689,11222620,12060778,16461910,2987848,11207367,19737352,19081080,17932079,19968790,19682252,18216012,2168872,26615189,27120414,27489185,27902860,28166228,28752667,29396913,30285297