AAA ATPase, replication initiation protein
product
replication initiation protein
Genomic Context
categories
[category|SW 3|Information processing] → [category|SW 3.1|Genetics] → [category|SW 3.1.1|DNA replication][category|SW 6|Groups of genes] → [category|SW 6.1|Essential genes][SW|Categories] containing this gene/protein
[SW|DNA replication], [SW|essential genes]This gene is a member of the following [SW|regulons]
[SW|Spo0A regulon]Gene
Coordinates on the chromosome (coding sequence)
410 -> 1,750
Phenotypes of a mutant
essential [Pubmed|12682299]The protein
Catalyzed reaction/ biological activity
binds multiple regions in the oriC region, required for recruitment of proteins needed to load the replicative helicase [protein|search|DnaC]Protein family
dnaA family (according to Swiss-Prot)[SW|Domains]
Effectors of protein activity
[protein|search|SirA] displaces [protein|search|DnaA] from the replication origin [Pubmed|19682252][protein|search|YabA] inhibits co-operative binding of DnaA to the ''oriC'' DNA [Pubmed|21895792]DnaA helix formation (and thus replication initiation) is inhibited by the interaction of either [protein|search|Soj], [protein|search|YabA] or [protein|search|DnaN] with the AAA domain of DnaA [Pubmed|23909787,22286949]interaction with [protein|search|DnaD] inhibits the ability of [protein|search|DnaA] to cooperatively bind to DNA [Pubmed|22821970]Structure
[PDB|2Z4R] (from ''Thermotoga maritima '') [Pubmed|18216012][SW|Localization]
throughout the cytoplasm [Pubmed|10844689][SW|Interactions]
[protein|search|DnaA] assembles into a right-handed helical oligomer built upon interactions between neighbouring AAA domains [Pubmed|23909787][protein|search|ParA]-[protein|search|DnaA] [Pubmed|22286949][protein|search|DnaA]-[protein|search|YabA] [Pubmed|23909787,12060778][protein|search|DnaA]-[protein|search|YabA]-[protein|search|DnaN] [Pubmed|12060778][protein|search|DnaA]-[protein|search|DnaD] [Pubmed|22821970,11222620][protein|search|SirA]-[protein|search|DnaA] (N-terminal domain) [Pubmed|25041308,19682252,21239581][protein|search|YqaH]-[protein|search|DnaA] [Pubmed|12060778][SW|DnaA]-[SW|Rok], to modulate [SW|Rok] activity [Pubmed|27902860]part of the [SW|primosome]: [protein|search|DnaA]-[protein|search|DnaG]-[protein|search|DnaC]-[protein|search|DnaI]-[protein|search|DnaD]-[protein|search|DnaB] [Pubmed|22797751]Expression and Regulation
Operon
''[protein|search|dnaA]-[protein|search|dnaN]'' [Pubmed|2987848][SW|Sigma factor]
[protein|search|SigA] [Pubmed|2987848]Regulation
negatively controlled by [protein|search|DnaA] [Pubmed|2168872] and [SW|Spo0A] [Pubmed|14651647]repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]Regulatory mechanism
[SW|Spo0A]: transcription repression [Pubmed|14651647]Additional information
term-seq has identified a potential novel regulatory RNA element including an intrinsic transcription terminator upstream of ''dnaA'' [Pubmed|27120414]Labs working on this gene/protein
[SW|Peter Graumann], Freiburg University, Germany [http://www.biologie.uni-freiburg.de/data/bio2/graumann/index.htm homepage][SW|Alan Grossman], MIT, Cambridge, MA, USA[SW|Heath Murray], Centre for Bacterial Cell Biology, Newcastle, UK [http://www.ncl.ac.uk/camb/staff/profile/heath.murray homepage]References
Reviews
20157337,21035377,21639790,22575476,22797751,26706151 The [SW|DnaA regulon]
Original publications
20511500,21097613,21239581,21895792,22286949,22821970,23909787,22396664,21911367,25041308,25340815,26020636,18854156,19011033,11222620,14651647,17140409,10844689,11222620,12060778,16461910,2987848,11207367,19737352,19081080,17932079,19968790,19682252,18216012,2168872,26615189,27120414,27489185,27902860 
