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satA [2019-01-20 17:16:43]
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satA [2019-01-20 17:16:43]

streptothricine acetyl-transferase
Locus
BSU40740
Isoelectric point
5.83
Molecular weight
20.25 kDa
Protein length
173 aa Sequence Blast
Gene length
519 bp Sequence Blast
Function
resistance to streptothricine
Product
streptothricine acetyl-transferase
Essential
no
Synonyms
yyaR
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Lifestyles → Coping with stress → Resistance against toxins/ antibiotics

    • Gene

    Coordinates
    4,185,160 → 4,185,681

    Phenotypes of a mutant

  • sensitive to streptothricine PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • acetylation of streptothricin at the expense of acetyl-CoA PubMed

    • Kinetic information

  • Km (streptothricine): 1 µM PubMed
  • Km (acetyl-CoA): 107 µM PubMed

    • Structure

  • 3PP9 (complex with acetyl-CoA, from B. anthracis, 35% identity, 57% similarity)

    • Biological materials

    Mutant

  • MGNA-B856 (yyaR::erm), available at the NBRP B. subtilis, Japan
  • BKE40740 (ΔsatA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCAATCTCCGCCAATC, downstream forward: _UP4_TAATTGATTATAAAACCTCA
  • BKK40740 (ΔsatA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATATCAATCTCCGCCAATC, downstream forward: _UP4_TAATTGATTATAAAACCTCA

    • References

    Research papers

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