SubtiBank SubtiBank
yflN [2018-11-07 16:16:14]
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yflN [2018-11-07 16:16:14]

similar to metallo-beta-lactamase
locus
BSU07620
pI
5.07
mw
28.68 kDa
protein length
264 aa Sequence Blast
gene length
792 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.1|Sporulation proteins] → [category|SW 4.2.1.5|Newly identified sporulation proteins (based on transcription profiling)]

    • Gene

    Coordinates
    835,740 → 836,534

    The protein

    Structure

  • [PDB|4AWY] (from Pseudomonas aeruginosa, 25% identity) [pubmed|22664968]

    • Expression and Regulation

    Operons

    genes
    [gene|C0DED68802EF78D2BC16507234AAC3B60D236E68|citM]-[gene|66B9809A77AB15E5C8C8E5A8E26752771AF55E3A|yflN]
    description
    [Pubmed|10972810]

    sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10972810], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

    • regulatory mechanism

  • [protein|1345FBA88CB55E0E897FEC8DA4D829B85A8393BB|CitT]: activation, [Pubmed|10972810], in [regulon|1345FBA88CB55E0E897FEC8DA4D829B85A8393BB|CitT regulon]
  • [protein|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA]: repression, [Pubmed|22900538,10972810], in [regulon|E39AFEB7B7F28969A35C4B51CC6E7819F71C79D9|CcpA regulon]

    • regulation

  • induced by citrate ([protein|search|CitT]) [Pubmed|10972810]
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    Biological materials

    Mutant

  • MGNA-C253 (yflN::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2251 NBRP B. subtilis, Japan]
  • BKE07620 (Δ[gene|66B9809A77AB15E5C8C8E5A8E26752771AF55E3A|yflN]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGACACCTCCGGAAT, downstream forward: _UP4_TGACACCCGCACCACGCGGG
  • BKK07620 (Δ[gene|66B9809A77AB15E5C8C8E5A8E26752771AF55E3A|yflN]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATAATGACACCTCCGGAAT, downstream forward: _UP4_TGACACCCGCACCACGCGGG

    • References

  • 10972810,22664968