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thdF [Tue Dec 13 2016 11:16:38 GMT+0100 (CET)]
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thdF [Tue Dec 13 2016 11:16:38 GMT+0100 (CET)]

GTP-binding protein, putative tRNA modification GTPase
locus
BSU41020
pI
4.00
mw
50.00 kDa
function
tRNA modification
product
putative tRNA modification GTPase
essential
no
synonyms
mnmE, trmE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.1|Translation] → [category|SW 3.3.1.8|tRNA modification and maturation]
  • [category|SW 6|Groups of genes] → [category|SW 6.3|GTP-binding proteins]

    • [SW|Categories] containing this gene/protein

  • [SW|translation], [SW|GTP-binding proteins], [SW|sporulation/ other]

    • This gene is a member of the following [SW|regulons]

  • [SW|ComK regulon], [SW|DnaA regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    4,211,510 -> 4,212,889

    Phenotypes of a mutant

  • inactivation of ''[SW|thdF]'' reduces sporulation efficiency to 21.9% that of wild type cells; longer mother cells, some with aberrant septa and forespores [Pubmed|26735940]

    • The protein

    Catalyzed reaction/ biological activity

  • Binds and hydrolyzes GTP and readily exchanges GDP for GTP

    • Protein family

  • MnmE subfamily (according to Swiss-Prot) Era/Obg family

    • Paralogous protein(s)

  • [protein|search|Era], [protein|search|Obg], [protein|search|YphC], [protein|search|YsxC], [protein|search|RbgA], [protein|search|YqeH], [protein|search|ThdF], [protein|search|YyaF], [protein|search|YnbA]

    • Structure

  • [PDB|1XZP] (from ''Thermotoga maritima'', 44% identity) [Pubmed|15616586]

    • [SW|Localization]

  • cytoplasm (according to Swiss-Prot)

    • [SW|Interactions]

  • [protein|search|GidA]-[protein|search|ThdF] (suggested by interaction of ''E. coli'' counterparts [Pubmed|19801413] and by co-localization on the chromosome)

    • Additional information

  • Information on the corresponding protein from ''E. coli'': [http://BioCyc.org/ECOLI/NEW-IMAGE?type=ENZYME&object=EG10997-MONOMER EcoCyc]
  • MnmE required for wild-type 5-methylaminomethyl-2-thiouridine modification of tRNA, interacts with [protein|search|GidA]. MnmE also appears to play a role in oxidation of thiophene and furan compounds and regulates glutamate-dependent acid resistance.

    • Expression and Regulation

    Operon

  • ''[protein|search|thdF]-[protein|search|gidA]-[protein|search|rsmG]-[protein|search|noc]'' [Pubmed|11807071]

    • Regulatory mechanism

  • [protein|search|ComK]: transcription activation [Pubmed|11948146]
  • [protein|search|DnaA]: transcription repression [Pubmed|26020636]

    • Labs working on this gene/protein

  • [SW|Naotake Ogasawara], Nara, Japan

    • References

    Reviews

  • 21885683

    • Original publications

  • 12427945,11807071,11948146,23630314,26020636,15616586,26735940