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thdF [2018-04-11 16:10:58]
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thdF [2018-04-11 16:10:58]

GTP-binding protein, putative tRNA modification GTPase
Locus
BSU41020
Isoelectric point
4.64
Molecular weight
50.81 kDa
Protein length
459 aa Sequence Blast
Gene length
1377 bp Sequence Blast
Function
tRNA modification
Product
putative tRNA modification GTPase
Essential
no
Synonyms
mnmE,trmE
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Translation → tRNA modification and maturation
  • Groups of genes → GTP-binding proteins

    • Gene

    Coordinates
    4,211,510 → 4,212,889

    Phenotypes of a mutant

  • inactivation of thdF reduces sporulation efficiency to 21.9% that of wild type cells; longer mother cells, some with aberrant septa and forespores PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • Binds and hydrolyzes GTP and readily exchanges GDP for GTP

    • Protein family

  • MnmE subfamily (according to Swiss-Prot) Era/Obg family

    • Paralogous protein(s)

  • Obg, YqeH, YnbA, YphC, YyaF, Era, YsxC, RbgA

    • Structure

  • 1XZP (from Thermotoga maritima, 44% identity) PubMed

    • Localization

  • cytoplasm (according to Swiss-Prot)

    • Additional information

  • Information on the corresponding protein from E. coli: EcoCyc
  • MnmE required for wild-type 5-methylaminomethyl-2-thiouridine modification of tRNA, interacts with GidA. MnmE also appears to play a role in oxidation of thiophene and furan compounds and regulates glutamate-dependent acid resistance.

    • Expression and Regulation

    Operons

    Genes
    thdF-gidA-gidB-noc
    Description
    PubMed

    Regulatory mechanism

  • ComK: activation, PubMed, in ComK regulon
  • DnaA: repression, PubMed, in DnaA regulon

    • Regulation

  • constitutively expressed PubMed
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    Biological materials

    Mutant

  • MGNA-B877 (thdF::erm), available at the NBRP B. subtilis, Japan
  • BKE41020 (ΔthdF::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTGTTCACCTCTCTTT, downstream forward: _UP4_TAATTAAAGGAGGAACTAGA
  • BKK41020 (ΔthdF::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGTTGTTCACCTCTCTTT, downstream forward: _UP4_TAATTAAAGGAGGAACTAGA

    • Labs working on this gene/protein

  • Naotake Ogasawara, Nara, Japan

    • References

    Reviews

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    Original publications

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