acsA [Thu Apr 21 2016 20:35:17 GMT+0200 (CEST)]

acetyl-CoA synthetase

Locus: BSU29680

Isoelectric point: 5.00

Molecular weight: 64.00 kDa

Function: utilization of acetate, fatty acids

Product: acetyl-CoA synthetase)

Essential: no

E.C.: 6.2.1.1

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Carbon metabolism → Utilization of specific carbon sources → Utilization of organic acids
• Metabolism → Lipid metabolism → Utilization of lipids → Utilization of fatty acids

Categories containing this gene/protein

• utilization of specific carbon sources, utilization of lipids

This gene is a member of the following regulons

• CcpA regulon, CodY regulon

Gene

Coordinates on the chromosome (coding sequence): 3,038,213 -> 3,039,931

The protein

Catalyzed reaction/ biological activity

• ATP acetate CoA = AMP diphosphate acetyl-CoA (according to Swiss-Prot)

Protein family

• ATP-dependent AMP-binding enzyme family (according to Swiss-Prot)

Modification

• acetylated on Lys-549 by AcuA, this results in inactivation PubMed, deacetylated by SrtN and AcuC deacetylates (and thereby activates) AcsA PubMed

Structure

• 2P2F (from Salmonella enterica, 37% identity) PubMed

Expression and Regulation

Operon

• acsA PubMed

Sigma factor

• SigA PubMed

Regulation

• repressed by glucose (4.5-fold) (CcpA) PubMed
• repressed during growth in the presence of branched chain amino acids (CodY) PubMed

Regulatory mechanism

• CcpA: transcription repression PubMed
• CodY: transcription repression PubMed

Biological materials

Mutant

• GP1212 (acsA::kan), available in Stülke lab

References

Reviews

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Original publications

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