SubtiBank SubtiBank
dnaE [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]
You are currently viewing an outdated version of SubtiWiki. Please use the newest version!

dnaE [Thu Oct 01 2015 13:51:44 GMT+0200 (CEST)]

DNA polymerase III (alpha subunit), part of the replisome
Locus
BSU29230
Isoelectric point
6.02
Molecular weight
125.00 kDa
Function
DNA replication
Product
DNA polymerase III (alpha subunit)
Essential
yes
E.C.
2.7.7.7
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
Loading

Categories containing this gene/protein

  • Information processing → Genetics → DNA replication
  • Groups of genes → Essential genes

    • Categories containing this gene/protein

  • DNA replication, essential genes

    • This gene is a member of the following regulons

  • LexA regulon

    • Gene

    Phenotypes of a mutant

  • essential PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1) (according to Swiss-Prot)
  • required for bacteriophage SPP1 replication PubMed

    • Protein family

  • DnaE subfamily (according to Swiss-Prot)

    • Structure

  • 3E0D (from Thermus aquaticus, 39% identity) PubMed

    • Localization

  • forms foci, the foci dissappear after the formation of DNA mismatches in a MutS-dependent manner or after DNA replication arrest PubMed
  • cytoplasm (according to Swiss-Prot)

    • Interactions

  • part of the replisome: PolC-HolA-HolB-DnaX-DnaN-DnaG-DnaC-DnaI-DnaD-SsbA-DnaE-PriA-DnaB PubMed
  • DnaE-MutL PubMed
  • DnaE-MutS PubMed
  • DnaE-DnaN PubMed
  • DnaE-SsbA PubMed
  • DnaE-DnaC PubMed
  • DnaE-DnaG PubMed
  • DnaE-HolA PubMed
  • DnaE-PolC PubMed

    • Expression and Regulation

    Operon

  • dnaE (according to DBTBS)

    • Regulation

  • induced by DNA damage (LexA) PubMed

    • Regulatory mechanism

  • LexA: transcription repression PubMed

    • References

    Reviews

    Loading

    Original publications

    Loading