yeeF [2020-03-05 09:58:19]

polymorphic toxin, non-specific metal-dependent DNase

locus: BSU_06812

pI: 8.94

mw: 74.00 kDa

protein length: 669 aa

gene length: 2010 bp

function: competetion with other bacteria

product: non-specific metal-dependent DNase

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.17|Toxins, antitoxins and immunity against toxins] → [category|SW 4.3.17.2|Type 2 TA systems]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.18|Toxins, antitoxins and immunity against toxins/ based on similarity]

Gene

Coordinates: 747,554 749,563

The protein

Catalyzed reaction/ biological activity

• non-specific metal-dependent DNase activity [pubmed|32117125]

[SW|Domains]

• [SW|LXG domain] (aa 1-235) (according to UniProt)

Expression and Regulation

Operons

• genes: [gene|655C07294D3A04763049D6701A7296BB4AF8E6B9|yeeF]-[gene|9202D58FAC1B7B9CF693D537A12C683A3573B17E|yezG]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-A963 (yeeF::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/963 NBRP B. subtilis, Japan]
• BKE06812 ([gene|655C07294D3A04763049D6701A7296BB4AF8E6B9|yeeF]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE06812 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACCATTTCCCTTCTTT, downstream forward: _UP4_TAGAAGAATGGAAACTAGGA
• BKK06812 ([gene|655C07294D3A04763049D6701A7296BB4AF8E6B9|yeeF]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK06812 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGACCATTTCCCTTCTTT, downstream forward: _UP4_TAGAAGAATGGAAACTAGGA

References

• 22200572,32117125