ycsD [2019-07-15 07:59:53]

-hydroxyacyl-(acyl carrier protein) dehydratase

locus: BSU_04030

pI: 4.69

mw: 14.11 kDa

protein length: 130 aa

gene length: 393 bp

function: fatty acid biosynthesis

product: -hydroxyacyl-ACP dehydratase

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]

Gene

Coordinates: 455,346 455,738

The protein

Protein family

• thioester dehydratase family (with [protein|BDA23FA513C240B07DC109BB62A7A5EB96B20965|YwpB], according to UniProt)
• FabZ subfamily (with [protein|BDA23FA513C240B07DC109BB62A7A5EB96B20965|YwpB], according to UniProt)

Paralogous protein(s)

• [protein|BDA23FA513C240B07DC109BB62A7A5EB96B20965|YwpB]

Structure

• [PDB|4I83]((3r)-hydroxymyristoyl-ACP dehydratase from Neisseria meningitidis, 34% identity)

Expression and Regulation

Operons

• genes: [gene|65506215AE58BF53C57599E566E4F78514DB45AA|ycsD]
  description: [pubmed|22383849]

Biological materials

Mutant

• MGNA-C021 (ycsD::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2019 NBRP B. subtilis, Japan]
• BKE04030 ([gene|65506215AE58BF53C57599E566E4F78514DB45AA|ycsD]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE04030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTATATCTCCTTTTTT, downstream forward: _UP4_TGAAAAAAACCCTTCACAAC
• BKK04030 ([gene|65506215AE58BF53C57599E566E4F78514DB45AA|ycsD]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK04030 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTTTATATCTCCTTTTTT, downstream forward: _UP4_TGAAAAAAACCCTTCACAAC

References

• 17919287