SubtiBank SubtiBank
yxjB [2018-09-25 13:56:15]
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yxjB [2018-09-25 13:56:15]

similar to 23S rRNA (guanine-N(1)-)-methyltransferase
locus
BSU39010
pI
8.13
mw
31.32 kDa
protein length
282 aa Sequence Blast
gene length
846 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.13|Resistance against toxins/ antibiotics]

    • Gene

    Coordinates
    4,004,288 → 4,005,136

    The protein

    Structure

  • [PDB|1P91] (from E. coli, 31% identity) [pubmed|14999102]

    • Expression and Regulation

    Operons

    genes
    [gene|65253C69BAE9679FE4475BFF76BBABBB0FA70C30|tlrB]
    description
    [Pubmed|10746760]

    regulatory mechanism

  • [protein|4910F1E5BAF274C828FD92C410A1B86FB1D358CE|TlrBL]: attenuation, [pubmed|31719185], in [regulon|4910F1E5BAF274C828FD92C410A1B86FB1D358CE|TlrBL regulon]

    • regulation

  • induced in the presence of tylosin (transcription attenuation and translation attenuation in the absence of tylosin) [Pubmed|31719185]
  • induction depends on [protein|43CFA3477B4EEB4CE0B2DF63ED2B5EDB171E79C7|NusG]-dependent [SW|RNA polymerase] pausing between the leader peptide [protein|4910F1E5BAF274C828FD92C410A1B86FB1D358CE|TlrBL] and [gene|65253C69BAE9679FE4475BFF76BBABBB0FA70C30|tlrB] coding sequences [Pubmed|31719185]

    • additional information

  • a [protein|8887ADC77C43F21CD375BDAA4D38E940786DAD4F|NusA]-dependent terminator and an SD-sequestering hairpin are present in the [gene|65253C69BAE9679FE4475BFF76BBABBB0FA70C30|tlrB] leader [Pubmed|31719185,27120414]
    • view in new tab

    Biological materials

    Mutant

  • MGNA-B726 (yxjB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1725 NBRP B. subtilis, Japan]
  • 1A995 ( ''yxjB''::''spec''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A995&Search=1A995 BGSC]
  • BKE39010 (Δ[gene|65253C69BAE9679FE4475BFF76BBABBB0FA70C30|tlrB]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE39010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAAAAAACACCCCAT, downstream forward: _UP4_TGAATGGTAGAGGAAAGCTC
  • BKK39010 (Δ[gene|65253C69BAE9679FE4475BFF76BBABBB0FA70C30|tlrB]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK39010 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATGATAAAAAACACCCCAT, downstream forward: _UP4_TGAATGGTAGAGGAAAGCTC

    • References

  • 10746760,27120414,14999102