ribU

riboflavin ECF transporter, S protein

Locus: BSU_23050

Isoelectric point: 10.12

Molecular weight: 20.41 kDa

Protein length: 190 aa

Gene length: 573 bp

Function: riboflavin uptake

Product: S protein of riboflavin ECF transporter

Essential: no

Synonyms: ypaA

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → ECF transporter
• Cellular processes → Transporters → ECF transporter → The substrate-specific S components of the ECF transporters
• Metabolism → Additional metabolic pathways → Biosynthesis of cofactors → Biosynthesis/ acquisition of riboflavin/ FAD
• Groups of genes → Membrane proteins

Gene

Coordinates: 2,410,017 2,410,589

The protein

Protein family

• prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family (single member, according to UniProt)

Structure

• 3P5N (RibU from Staphylococcus aureus)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: ribU
  Description: PubMed

  Regulatory mechanism

  • FMN-box: transcription termination, via MgtE riboswitch, sequestration of the Shine-Dalgarno sequence in the presence of FMN or FMNH₂, this is counter-acted upon binding of RibR, in FMN-box

  Regulation

  • expressed in the absence of FMN (FMN-box) PubMed

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant

• MGNA-A478 (ypaA::erm), available at the NBRP B. subtilis, Japan
• BKE23050 (ribU::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTCATTCTCTCCTTTTC, downstream forward: _UP4_TAAATAAAAAAGCTCCCGTT
• BKK23050 (ribU::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CACTTCATTCTCTCCTTTTC, downstream forward: _UP4_TAAATAAAAAAGCTCCCGTT

References

Reviews

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Original publications

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