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ribU
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ribU

riboflavin [SW|ECF transporter], S protein
locus
BSU_23050
pI
10.12
mw
20.41 kDa
protein length
190 aa Sequence Blast
gene length
573 bp Sequence Blast
function
riboflavin uptake
product
S protein of riboflavin [SW|ECF transporter]
essential
no
synonyms
ypaA
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter]
  • [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.3|ECF transporter] → [category|SW 1.2.3.2|The substrate-specific S components of the ECF transporters]
  • [category|SW 2|Metabolism] → [category|SW 2.6|Additional metabolic pathways] → [category|SW 2.6.2|Biosynthesis of cofactors] → [category|SW 2.6.2.2|Biosynthesis/ acquisition of riboflavin/ FAD]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    2,410,017 2,410,589

    The protein

    Protein family

  • prokaryotic riboflavin transporter (P-RFT) (TC 2.A.87) family (single member, according to UniProt)

    • Structure

  • [PDB|3P5N] (RibU from ''Staphylococcus aureus'')

    • [SW|Localization]

  • cell membrane (according to UniProt)

    • Expression and Regulation

    Operons

    genes
    [gene|651C370386E9FB45E3B98001800D342FEDB43E9A|ribU]
    description
    [Pubmed|21815947]

    regulatory mechanism

  • [regulon|FMN-box|FMN-box]: transcription termination, via [protein|C245476CE35028167510F67153F392D91447553F|MgtE riboswitch], sequestration of the Shine-Dalgarno sequence in the presence of FMN or FMNH2, this is counter-acted upon binding of [protein|3D32B6C1DC9CAC615B02E14EA846648C3B0E61D6|RibR], in [regulon|FMN-box|FMN-box]

    • regulation

  • expressed in the absence of FMN ([protein|search|FMN-box]) [Pubmed|17693491]

    • additional information

  • the mRNA is substantially stabilized upon depletion of [gene|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] [PubMed|21815947]
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    Biological materials

    Mutant

  • MGNA-A478 (ypaA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/478 NBRP B. subtilis, Japan]
  • BKE23050 ([gene|651C370386E9FB45E3B98001800D342FEDB43E9A|ribU]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE23050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTCATTCTCTCCTTTTC, downstream forward: _UP4_TAAATAAAAAAGCTCCCGTT
  • BKK23050 ([gene|651C370386E9FB45E3B98001800D342FEDB43E9A|ribU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK23050 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CACTTCATTCTCTCCTTTTC, downstream forward: _UP4_TAAATAAAAAAGCTCCCGTT

    • References

    Reviews

  • 20497229,22574898,24362466

    • Original publications

  • 12456892,20972419,17693491,21815947,24356467,24755604,25438558,26494285,27312125