des [2017-8-11 16:0:3]

phospholipid desaturase

locus: BSU19180

pI: 9.88

mw: 40.55 kDa

protein length: 352 aa

gene length: 1056 bp

function: adaptation of membrane fluidity at low temperatures

product: phospholipid desaturase

essential: no

synonyms: yocE

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.3|Lipid metabolism/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.5|Cold stress proteins]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 2,089,396 → 2,090,454

Phenotypes of a mutant

• cold-sensitive [Pubmed|19820084]

The protein

Catalyzed reaction/ biological activity

• introduces double bonds into long chain fatty acids [Pubmed|21665975]

[SW|Cofactors]

• [protein|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|Des] receives electrons from ferredoxin ([protein|4164F07C581320D67FDC1ED5014272B81702A371|Fer]) or from either of the two flavodoxins, [protein|0932DDC285635C02067A3302CEDA302FE4269179|YkuN] or [protein|ECAFD2873ADE6B65C7B3C27FF26E0FAAA74839CC|YkuP], the three proteins can functionally replace each other [Pubmed|21665975]

[SW|Localization]

• integral membrane protein [Pubmed|21665975]

Expression and Regulation

Operons

• genes: [gene|644C0C354B72FC07222EE45F4D2E0E57434B5EB5|des]
  description: [Pubmed|10559169]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|10559169], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|DesR]: activation, [Pubmed|11285232], [Pubmed|19820084], in [regulon|F08BE4ACC0ECE1416B012CA72B027423D4D9B00A|DesR regulon]

  regulation

  • induced upon cold shock ([protein|search|DesR]) [Pubmed|11285232]

  additional information

  • in strains evolved for growth at low pressure, 'des' expression is significantly increased as compared to the wild type [PubMed|22416764]

Biological materials

Mutant

• MGNA-A319 (yocE::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/319 NBRP B. subtilis, Japan]
• 1A835 ( ''des''::''kan''), [Pubmed|11285232], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A835&Search=1A835 BGSC]

Labs working on this gene/protein

• [SW|Diego de Mendoza], Universidad Nacional de Rosario, Argentine [http://www.ibr.gov.ar/eng/investigacion/demendoza.htm homepage]

References

Reviews

• 19363032

Original publications

• 19164152,11251847,12399512,9555904,10559169,11285232,12207704,12730185,19820084,12207704,20581210,22416764,18154726,21665975,26647299,28461449