glnR [Sat Jun 25 2016 17:09:19 GMT+0200 (CEST)]

transcriptional repressor (MerR family) of the glnR-glnA operon

locus: BSU17450

pI: 9.00

mw: 15.00 kDa

function: regulation of glutamine synthesis

product: transcription repressor (MerR family)

essential: no

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.1|Biosynthesis/ acquisition of glutamate/ glutamine/ ammonium assimilation]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

[SW|Categories] containing this gene/protein

• [SW|biosynthesis/ acquisition of amino acids], [SW|glutamate metabolism], [SW|transcription factors and their control]

This gene is a member of the following [SW|regulons]

• [SW|GlnR regulon], [SW|TnrA regulon]

Gene

Coordinates on the chromosome (coding sequence): 1,877,959 -> 1,878,366

The protein

Protein family

• [SW|MerR family]

[SW|Cofactors]

• feedback-inhibited [protein|search|GlnA] [Pubmed|18195355]

Effectors of protein activity

• activity is enhanced upon interaction of the C-terminal domain with [protein|search|GlnA] [Pubmed|18331450]

Structure

• [PDB|4R4E] (the GlnR-DNA complex) [Pubmed|25691471]

[SW|Interactions]

• [protein|search|GlnR]-[protein|search|GlnA], with feedback-inhibited [protein|search|GlnA], this results in DNA binding [Pubmed|18195355]

Expression and Regulation

Operon

• ''[protein|search|glnR]-[protein|search|glnA]'' [Pubmed|8636055]

[SW|Sigma factor]

• [protein|search|SigA] [Pubmed|2906311]

Regulation

• expressed in the absence of glutamine ([protein|search|GlnR]) [Pubmed|8636055]
• repressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|8799114]

Regulatory mechanism

• [protein|search|GlnR]-[protein|search|GlnA] complex: transcription repression [Pubmed|8636055]
• [protein|search|TnrA]: transcription repression [Pubmed|8799114]

Biological materials

Mutant

• BP148 (del(''[protein|search|glnR]-[protein|search|glnA]'')::''cat''), available in [SW|Fabian Commichau]'s lab

lacZ fusion

• pGP189 (in [protein|search|pAC7]), available in [SW|Jörg Stülke]'s lab

Labs working on this gene/protein

• [SW|Susan Fisher], Boston, USA [http://www.bumc.bu.edu/microbiology/research/susan-h-fisher-phd/ homepage]

References

Reviews

• 22625175

Original publications

• 12374841,9287005,8799114,18195355,2573733,8636055,19233925,2906311,18331450,16547045,23535029,25691471,25755103