yfmS [2020-04-13 16:35:37]

soluble chemotaxis receptor

locus: BSU_07360

pI: 4.53

mw: 30.17 kDa

protein length: 286 aa

gene length: 861 bp

function: control of chemotaxis

product: soluble chemotaxis receptor

essential: no

synonyms

Genomic Context

categories

• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.1|Motility and chemotaxis] → [category|SW 4.1.1.1|Signal transduction in motility and chemotaxis] → [category|SW 4.1.1.1.3|Soluble chemoreceptors]

Gene

Coordinates: 808,562 809,422

The protein

[SW|Domains]

• [SW|Methyl-accepting transducer domain] (aa 68-268) (according to UniProt)

[SW|Localization]

• forms clusters at the cell poles [Pubmed|21515776]

Expression and Regulation

Operons

• genes: [gene|EF0AAAF5BAE8E1F54FCA296643F7B9E3CE257B1D|yfmT]-[gene|63BCB42FC501B57ABC9FF252AC5D04BB729EA6B9|yfmS]
  description: [Pubmed|9141694,15033535]

  sigma factors

  • [protein|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD]: sigma factor, [Pubmed|15033535], in [regulon|7024E4162A6D827069F882FDEACA696EBC05DD40|SigD regulon]

  additional information

  • in minimal medium, YfmS is present with 4,200 +/- 800 molecules per cell [PubMed|21515776]

Biological materials

Mutant

• MGNA-C233 (yfmS::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2231 NBRP B. subtilis, Japan]
• BKE07360 ([gene|63BCB42FC501B57ABC9FF252AC5D04BB729EA6B9|yfmS]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE07360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTCAATTCCATTCACTCCT, downstream forward: _UP4_TAAGAGACCGGGGACAAACA
• BKK07360 ([gene|63BCB42FC501B57ABC9FF252AC5D04BB729EA6B9|yfmS]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK07360 BGSC], [Pubmed|28189581], upstream reverse: _UP1_GGTCAATTCCATTCACTCCT, downstream forward: _UP4_TAAGAGACCGGGGACAAACA

References

• 9141694,15033535,21515776