proG [2019-07-31 11:15:29]

1-pyrroline-5-carboxylate dehydrogenase

locus: BSU_12910

pI: 4.91

mw: 30.05 kDa

protein length: 272 aa

gene length: 819 bp

function: biosynthesis of proline

product: 1-pyrroline-5-carboxylate dehydrogenase

essential: no

ec: 1.5.1.2

synonyms: ykeA,yzcA

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.2|Biosynthesis/ acquisition of proline]

Gene

Coordinates: 1,359,454 1,360,272

The protein

Catalyzed reaction/ biological activity

• 1-pyrroline-5-carboxylate + NADPH + H⁺ --> L-proline + NADP⁺ [pubmed|28824574]

Protein family

• [SW|pyrroline-5-carboxylate reductase family] [pubmed|28824574]

[SW|Cofactors]

• NADPH [pubmed|28824574]

Effectors of protein activity

• feedback-inhibited by proline [pubmed|28824574]

[SW|Localization]

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• genes: [gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]
  description: [Pubmed|1766370,21784929]

Biological materials

Mutant

• MGNA-B308 (ykeA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1307 NBRP B. subtilis, Japan]
• BKE12910 ([gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE12910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGACTCAGTCCTTTCAT, downstream forward: _UP4_TGAATCCTTGAAAGAGGATT
• BKK12910 ([gene|63AFCEE5DEA6B2604C02592C5FB52EDFAE68913E|proG]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK12910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCGACTCAGTCCTTTCAT, downstream forward: _UP4_TGAATCCTTGAAAGAGGATT

References

Reviews

• 25367752

Original publications

• 11418582,1766370,21784929,28752945,28824574