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phoP [2018-10-26 12:05:18]
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phoP [2018-10-26 12:05:18]

two-component response regulator (OmpR family), regulation of phosphate metabolism
Locus
BSU29110
Isoelectric point
5.07
Molecular weight
27.53 kDa
Protein length
240 aa Sequence Blast
Gene length
720 bp Sequence Blast
Function
regulation of phosphate metabolism (phoA, phoB, phoD, resABCDE, tagA-tagB, tagDEF, [tuaA-H])
Product
two-component response regulator (OmpR family)
Essential
no
Synonyms
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Metabolism → Additional metabolic pathways → Phosphate metabolism
  • Information processing → Regulation of gene expression → Transcription factors and their control → Two-component system response regulators
  • Information processing → Regulation of gene expression → Regulators of core metabolism
  • Lifestyles → Sporulation → Sporulation proteins → Sporulation proteins/ other
  • Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
  • Groups of genes → Phosphoproteins → Phosphorylation on an Asp residue

    • Gene

    Coordinates
    2,977,800 → 2,978,522

    The protein

    Protein family

  • OmpR family of two-component response regulators

    • Paralogous protein(s)

  • YkoG, YclJ, WalR

    • Modification

  • phosphorylation by PhoR under conditions of phosphate limitation (stimulates DNA-binding activity)

    • Effectors of protein activity

  • phosphorylation stimulates DNA-binding activity

    • Structure

  • 2OQR (from Mycobacterium tuberculosis, 44% identity) PubMed
  • 1MVO (receiver domain)

    • Localization

  • cell membrane (according to Swiss-Prot)

    • Additional information

  • information on binding sites can be found in the PRODORIC2 database

    • Expression and Regulation

    Operons

    Genes
    phoP-phoR
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon
  • SigB: sigma factor, PubMed, in SigB regulon
  • SigE: sigma factor, PubMed, in SigE regulon

    • Regulatory mechanism

  • CcpA: repression, PubMed, in CcpA regulon
  • ScoC: repression, PubMed, in ScoC regulon
  • PhoP: activation, due to PhoP~P binding to the 3’end of the PhoP-PhoR operon PubMed, in PhoP regulon

    • Regulation

  • carbon catabolite repression (CcpA) PubMed
    • view in new tab

    Biological materials

    Mutant

  • 1A966 ( phoP::tet), PubMed, available at BGSC
  • BKE29110 (ΔphoP::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTGTGCCTCCAGTATT, downstream forward: _UP4_TATAAACTGGAGGAGCCAAA
  • BKK29110 (ΔphoP::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGCTGTGCCTCCAGTATT, downstream forward: _UP4_TATAAACTGGAGGAGCCAAA

    • Labs working on this gene/protein

  • Marion Hulett, University of Illinois at Chicago, USA Homepage

    • References

    Regulation of phoP]-[[gene|phoR] expression

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    Biochemical analyses

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    Targets of PhoR

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    Other original publications

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