rpoE [2020-08-25 13:04:17]

[protein|search|RNA polymerase] delta subunit, affects the regulation of [SW|RNA polymerase] by the concentration of the initiating nucleoside triphosphate (iNTP)

locus: BSU_37160

pI: 3.65

mw: 20.25 kDa

protein length: 173 aa

gene length: 522 bp

function: [SW|transcription]

product: [SW|RNA polymerase] delta subunit

essential: no

ec: 2.7.7.6

synonyms

Genomic Context

categories

• [category|SW 3|Information processing] → [category|SW 3.2|RNA synthesis and degradation] → [category|SW 3.2.1|Transcription] → [category|SW 3.2.1.1|RNA polymerase]

Gene

Coordinates: 3,812,542 3,813,063

Phenotypes of a mutant

• [protein|632C661255DD9C2865CB2F9D68A805997A833BCB|RpoE] is essential for cell survival when facing a competing strain in changing environment [Pubmed|23543716]

The protein

Catalyzed reaction/ biological activity

• binds to DNA immediately upstream of the promoter element at A-rich sequences on the abrB and rrnB1 promoters and facilitates open complex formation [Pubmed|26546673]
• represses expression of ''[gene|29D91115BB3AC03538D618E54A5A57F92777EFF6|spo0B]'' [Pubmed|27679485]

Protein family

• rpoE family (single member, according to UniProt)

[SW|Domains]

• HARE-HTH domain (aa 15-81) (according to UniProt)

Structure

• [PDB|2M4K] (full-length protein) [Pubmed|23868186]
• [PDB|2KRC] (N-terminal domain) [Pubmed|20310067]

[SW|Localization]

• closely associated with [SW|RNA polymerase] involved in transcribing both mRNA and rRNA operons [Pubmed|20724389]

Expression and Regulation

Operons

• genes: [gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]-[gene|CEE04B9FED63AFEAB912791402F7578E852005D1|pyrG]
  description: [Pubmed|29794222]

  regulation

  • the mRNA is processed between [gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE] and [gene|CEE04B9FED63AFEAB912791402F7578E852005D1|pyrG] by [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y], this requires the [protein|EE52DFA35B935E551871D079A9BE877DB2001A3B|YmcA]-[protein|6C9A092F38739A3759793EF8B496569CD02C2E3F|YlbF]-[protein|EBD15C174A03B7FCDFFE4C5DB5D86E93F1B9CAC4|YaaT] complex [Pubmed|29794222]

• genes: [gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]
  description: [Pubmed|10336502]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|17189250], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

• genes: [gene|DEE0DA15EE9EA27BA8C332204E40D336324A111C|fadF]-[gene|7D89BBB52403BF99416250688EFA90C2BE8EB591|acdA]-[gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]
  description: [Pubmed|17189250]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|17189250], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]

  regulatory mechanism

  • [protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]: repression, [Pubmed|17189250], in [regulon|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR regulon]

  regulation

  • repressed in the absence of long-chain fatty acids ([protein|8A8F93BAD0582F712C9EE1C3FE6C04292E91F390|FadR]) [Pubmed|17189250]

additional information

• present at equimolar levels with [SW|RNA polymerase] [PubMed|20724389]

Biological materials

Mutant

• BKE37160 ([gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE37160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGGTCGGACACTCCCTTT, downstream forward: _UP4_TAGTATCAGATTTCATCTTC
• BKK37160 ([gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK37160 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CAAGGTCGGACACTCCCTTT, downstream forward: _UP4_TAGTATCAGATTTCATCTTC
• LK1098 (([gene|632C661255DD9C2865CB2F9D68A805997A833BCB|rpoE]::aphA3), available in [SW|Libor Krasny]'s and [SW|Jörg Stülke]'s labs

lacZ fusion

• BP1145: Promotor-lacZ fusion, available at [SW|Jörg Stülke]'s and [SW|Fabian Commichau]'s labs

labs

• [SW|Arthur Aronson], Purdue University, West Lafayette, USA [http://wwwdev.gradschool.purdue.edu/PULSe/faculty.cfm?fid=5&range=0 homepage]

References

Reviews

• 22210308,25878038

Original publications

• 7545758,7599136,7515111,10336502,2843435,17189250,3097010,6788769,23543716,22350953,20890634,20724389,20310067,23868186,21424579,24520113,24937760,24491578,26546673,27679485,29794222