ahrC [2017-08-25 17:09:20]

transcriptional regulator of the ArgR family, AhrC represses the genes for arginine biosynthesis and activates the genes for arginine catabolism.

locus: BSU24250

pI: 5.52

mw: 16.69 kDa

protein length: 149 aa

gene length: 447 bp

function: transcriptional regulator of arginine metabolic genes

product: transcriptional regulator (ArgR family)

essential: no

synonyms: argR

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.4|Biosynthesis/ acquisition of arginine]
• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.4|Utilization of arginine/ ornithine]
• [category|SW 3|Information processing] → [category|SW 3.4|Regulation of gene expression] → [category|SW 3.4.2|Transcription factors and their control] → [category|SW 3.4.2.5|Transcription factors/ other]

Gene

Coordinates: 2,522,324 → 2,522,773

Phenotypes of a mutant

• increased intracellular concentration of ornithine, citrulline, and arginine [pubmed|28679749]
• [gene|search|ahrC ]inactivation allows growth of a [gene|search|ktrA ][gene|search|ktrB ][gene|search|kimA ]mutant at low potassium concentration [pubmed|28679749]

The protein

Catalyzed reaction/ biological activity

• transcriptional activator/ repressor of genes involved in arginine metabolism

Protein family

• [SW|ArgR family]

[SW|Cofactors]

• L-arginine is the co-factor required for transcription repression/ activation

Structure

• [PDB|2P5L] (complex with an 18bp DNA operator)
• [PDB|2P5K] (N-terminus)
• [PDB|2P5M] (C-Terminus)
• [PDB|1F9N], [Pubmed|18455186]

[SW|Localization]

• Cytoplasm

Expression and Regulation

Operons

• genes: [gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]
  description: [Pubmed|2106508]

  regulation

  • by [protein|search|sRNA] [protein|search|sr1]

  additional information

  • expression is fourfold increased upon depletion of ''[SW|nusA]'' [http://www.nature.com/articles/nmicrobiol20157 Reference]

• genes: [gene|EA9FCF84AE4FA80993566B62A9200D4B32AF5670|dxs]-[gene|923C4177500E9678A0B4855F8F481D4A36ECD863|yqxC]-[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]-[gene|5680A06A74D3EC6310EF8677795511C75DB3059E|recN]
  description: [pubmed|22383849]

  regulatory mechanism

  • [regulon|stringent response|stringent response]: negative regulation, [pubmed|11948165], in [regulon|stringent response|stringent response]

  regulation

  • [protein|CEE73284EFC0DBE8870CE0B474922DED79475A57|RelA] dependent downregulation (Class I) during stringent response [Pubmed|11948165]

Other regulations

• [protein|FC93BA60BD185DE5EF31D5605F0B8294EC2E8E5A|Sr1]: translation inhibition,

Biological materials

Mutant

• GP729 (ahrC::aphA3), available in [SW|Jörg Stülke]'s lab
• GP2185 (Δ[gene|62F8E0D6BC9DA6390256FA8FB684EA5C9C722AE4|ahrC]::''ermC''), available in [SW|Jörg Stülke]'s lab [pubmed|28679749]

Labs working on this gene/protein

• [SW|Simon Phillips], Leeds University, UK [http://www.astbury.leeds.ac.uk/People/staffpage.php?StaffID=SEVP Homepage]
• [SW|Michel Debarbouille], Pasteur Institute, Paris, France [http://www.pasteur.fr/ip/easysite/go/03b-00000m-0ob/recherche/departements-scientifiques/microbiologie/unites-et-groupes/unite-de-biologie-des-bacteries-pathogenes-a-gram-positif/les-membres-de-l-equipe Homepage]

References

• 17020585,17576690,18455186,18007040,18007039,11856827,11305941,9383188,7565595,1312212,2106508,27171430,28679749