pbuO

similar to hypoxanthine/guanine permease PbuG

Locus: BSU_29990

Isoelectric point: 7.48

Molecular weight: 45.26 kDa

Protein length: 432 aa

Gene length: 1299 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ytiP

Genomic Context

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Categories containing this gene/protein

• Cellular processes → Transporters → Transporters/ other → Nucleotide/ nucleoside transporter
• Metabolism → Nucleotide metabolism → Biosynthesis/ acquisition of nucleotides → Biosynthesis/ acquisition of purine nucleotides/ based on similarity

Gene

Coordinates: 3,068,908 3,070,206

The protein

Protein family

• xanthine/uracil permease family (according to UniProt)

Paralogous protein(s)

• PbuG

Expression and Regulation

Operons

• Genes: pbuO
  Description: PubMed

  Regulatory mechanism

  • PurR: repression, (molecular inducer: PRPP) PubMed, in PurR regulon

  Regulation

  • repressed in the presence of purine nucleotides (PurR) PubMed

Biological materials

Mutant

• MGNA-A819 (ytiP::erm), available at the NBRP B. subtilis, Japan
• BKE29990 (pbuO::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGTATCCTCCAATTACG, downstream forward: _UP4_TAAGGATAGACCAAAAAACC
• BKK29990 (pbuO::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATAGTATCCTCCAATTACG, downstream forward: _UP4_TAAGGATAGACCAAAAAACC

References

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