SubtiBank SubtiBank
alaT [2018-02-10T16:51:38.000Z]
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alaT [2018-02-10T16:51:38.000Z]

similar to PLP-dependent methionine aminotransferase
locus
BSU31400
pI
5.11
mw
42.15 kDa
protein length
386 aa Sequence Blast
gene length
1158 bp Sequence Blast
function
unknown
product
unknown
essential
no
synonyms
yugH
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.1|Biosynthesis/ acquisition of amino acids] → [category|SW 2.3.1.15|Biosynthesis/acquisition of L- and D-alanine]

    • Gene

    Coordinates
    3,225,772 → 3,226,932

    The protein

    Paralogous protein(s)

  • [protein|094AE3AC8DFAFC8048ADAAB34F76DF2562D80CE7|PatA](45%), [protein|8F7432F7EF0F767C6000D553A3ACE29FBA2EDCEE|YhdR]

    • Structure

  • [PDB|1DJU] (from ''Pyrococcus horikoshii'', 47% identity) [Pubmed|10671523]

    • Additional information

  • The gene is annotated in KEGG as an ortholog of N-succinyldiaminopimelate aminotransferase EC 2.6.1.17. It is annotated in Swiss-Prot as “alanine transaminase”. In MetaCyc the gene is marked as “similar to aspartate
  • aminotransferase”. No literature/experimental evidence supporting the annotation is available. [Pubmed|19935659]

    • Expression and Regulation

    Operons

    genes
    [gene|1CA87275AFA1B4E4B5E8E10229009F785E70BBA8|alaR]-[gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT]
    description
    [Pubmed|22383849]

    additional information

  • A [protein|search|ncRNA] is predicted between [gene|B0908BB4B8B569A7A78BB0B84784FD4C427C598B|yugI] and [gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT] [PubMed|20525796]
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    Biological materials

    Mutant

  • MGNA-A621 (yugH::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/621 NBRP B. subtilis, Japan]
  • BKE31400 (Δ[gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE31400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATAGTCTGATAAATACGAAG, downstream forward: _UP4_TAAAAAAAGATACGAACCTT
  • BKK31400 (Δ[gene|62A9CD9AF72D0A48EC5ABC1585E2D8636D36D021|alaT]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK31400 BGSC], [Pubmed|28189581], upstream reverse: _UP1_ATAGTCTGATAAATACGAAG, downstream forward: _UP4_TAAAAAAAGATACGAACCTT

    • References

  • 20525796,22383849,10671523