tagU

phosphotransferase, attaches teichoic acid, teichuronic acid, or acidic capsular polysaccharide to cell wall peptidoglycan via phosphodiester linkage to N-acteylmuramic acid

locus: BSU_35650

pI: 9.53

mw: 34.43 kDa

protein length: 306 aa

gene length: 921 bp

function: transfer of anionic cell wall polymers from lipid-linked precursors to peptidoglycan

product: phosphotransferase, responsible for attachment of anionic polymers to peptidoglycan

essential: no

synonyms: lytR

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.1|Cell wall synthesis] → [category|SW 1.1.1.6|Export of anionic polymers and attachment to peptidoglycan]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 3,663,281 3,664,201

Phenotypes of a mutant

• a ''[gene|62A3CC548E033A45F94FE4386EE29711A6A14C6B|tagU]'' mutant has no phenoype, the triple ''[gene|68FE85BAA457199D34C9068F38756972CD2D280D|tagT] [gene|62A3CC548E033A45F94FE4386EE29711A6A14C6B|tagU] [gene|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|tagV]'' mutant is unable to grow under normal conditions [Pubmed|21964069]

The protein

Protein family

• LytR/CpsA/Psr (LCP) family (with [protein|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|TagV] and [protein|68FE85BAA457199D34C9068F38756972CD2D280D|TagT], according to UniProt) [Pubmed|19099556]

Paralogous protein(s)

• [protein|68FE85BAA457199D34C9068F38756972CD2D280D|TagT], [protein|E244A5ABB2FBD4FC3880F0DB700BD935991AE6DD|TagV]

[SW|Cofactors]

• Mg²⁺ [Pubmed|29107701,21964069]

Structure

• [PDB|6UF6] (aa 62-306) [pubmed|31969390]
• [PDB|3MEJ] ([protein|68FE85BAA457199D34C9068F38756972CD2D280D|TagT], 36% identity, 69% similarity)

[SW|Localization]

• cytoplasmic membrane (with extracytoplasmic catalaytic domain) [Pubmed|21964069]

Expression and Regulation

Operons

• genes: [gene|62A3CC548E033A45F94FE4386EE29711A6A14C6B|tagU]
  description: [Pubmed|1357079]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA]: sigma factor, [Pubmed|1357079,9636707], in [regulon|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|SigA regulon]
  • [protein|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM]: sigma factor, [Pubmed|19047346], in [regulon|081DF3EE9FA56209D648C7677188C61CE3AA8E41|SigM regulon]
  • [protein|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX]: sigma factor, [Pubmed|1357079,9636707], in [regulon|E77364F274A520FCCA9F5C9504E317B047BA29E6|SigX regulon]

  regulatory mechanism

  • [protein|62A3CC548E033A45F94FE4386EE29711A6A14C6B|TagU]: auto-repression, [Pubmed|1357079], in [regulon|62A3CC548E033A45F94FE4386EE29711A6A14C6B|TagU regulon]

additional information

• the mRNA is cleaved by [protein|BAB297D18F94FFA67B8D12A684AB4D1BCDFB4981|RNase III] [PubMed|26883633]

Biological materials

Mutant

• BKE35650 ([gene|62A3CC548E033A45F94FE4386EE29711A6A14C6B|tagU]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE35650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTTTGCACCTCGTCTG, downstream forward: _UP4_TAAAACAAAAAGAAGCTTCG
• BKK35650 ([gene|62A3CC548E033A45F94FE4386EE29711A6A14C6B|tagU]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK35650 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCCTTTGCACCTCGTCTG, downstream forward: _UP4_TAAAACAAAAAGAAGCTTCG

References

Reviews

• 24024634

Original publications

• 9636707,1357079,19099556,21964069,26883633,29107701,31969390