yybJ

similar to [SW|ABC transporter ](ATP-binding protein)

locus: BSU_40620

pI: 8.68

mw: 24.13 kDa

protein length: 218 aa

gene length: 657 bp

function: unknown

product: [SW|ABC transporter ](ATP-binding protein)

essential: no

synonyms

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.2|Transporters] → [category|SW 1.2.1|ABC transporters] → [category|SW 1.2.1.2|Exporters] → [category|SW 1.2.1.2.6|Exporters of unknown function]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

Gene

Coordinates: 4,175,869 4,176,525

The protein

Protein family

• [SW|ABC transporter superfamily] (according to UniProt)

[SW|Domains]

• [SW|ABC transporter domain] (aa 2-216) (according to UniProt)

Structure

• [PDB|1VPL] (from Thermotoga maritima, 35% identity)

Expression and Regulation

Operons

• genes: [gene|5A68286ADFB1B334DC626612C74D03AE4DBAF0A7|yybN]-[gene|BA4CB875FD72C7E7767AFA44A5D8C32448F5E18C|yybM]-[gene|CE98D731B8D9BDBF4EB119346BB59F5E5C48C827|yybL]-[gene|8950AD10BE5B6EDA74C341D272201D1BA9DBAC85|yybK]-[gene|6260516A5ED4A49457997592F29CEC10B7A2CCA7|yybJ]
  description: [pubmed|22383849]

  regulatory mechanism

  • [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]: repression, [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]-[protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] [Pubmed|27902860,15743949], in [regulon|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok regulon]
  • [protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA]: repression, [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]-[protein|6740108089F13116F200C15F35C2E7561E990FEB|DnaA] [Pubmed|27902860,15743949], in [regulon|6740108089F13116F200C15F35C2E7561E990FEB|DnaA regulon]

  regulation

  • repressed by [protein|1508770D5BCA5739D13E3ABD11CC5C103C07FF25|Rok]-[SW|DnaA] [Pubmed| 27902860,15743949]

Biological materials

Mutant

• MGNA-B844 (yybJ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1843 NBRP B. subtilis, Japan]
• BKE40620 ([gene|6260516A5ED4A49457997592F29CEC10B7A2CCA7|yybJ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE40620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTGTTAGGTTTAATACCT, downstream forward: _UP4_TAGTTTAAACTGGCTATTTC
• BKK40620 ([gene|6260516A5ED4A49457997592F29CEC10B7A2CCA7|yybJ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK40620 BGSC], [Pubmed|28189581], upstream reverse: _UP1_TTTTGTTAGGTTTAATACCT, downstream forward: _UP4_TAGTTTAAACTGGCTATTTC

References

• 10092453,23199363,27902860