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gndA [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]
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gndA [Tue Dec 13 2016 11:16:34 GMT+0100 (CET)]

NADP-dependent phosphogluconate dehydrogenase
locus
BSU23860
pI
5.00
mw
51.00 kDa
function
pentose phosphate pathway
product
NADP-dependent phosphogluconate dehydrogenase
essential
no
ec
1.1.1.44
synonyms
yqjI
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.1|Carbon core metabolism] → [category|SW 2.2.1.3|Pentose phosphate pathway]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.6|Phosphorylation on a Thr residue]

    • [SW|Categories] containing this gene/protein

  • [SW|carbon core metabolism], [SW|phosphoproteins], [SW|most abundant proteins]

    • Gene

    Coordinates on the chromosome (coding sequence)
    2,480,750 -> 2,482,159

    The protein

    Catalyzed reaction/ biological activity

  • 6-phospho-D-gluconate NADP = D-ribulose 5-phosphate CO2 NADPH (according to Swiss-Prot)

    • Protein family

  • 6-phosphogluconate dehydrogenase family (according to Swiss-Prot)

    • Paralogous protein(s)

  • [protein|search|GntZ]

    • Kinetic information

  • Reversible reaction [Pubmed|15231785]

    • Modification

  • phosphorylation on (Thr-15 OR Thr-17) [Pubmed|17218307]

    • Structure

  • [PDB|2W8Z] (from ''Geobacillus stearothermophilus'', 83% identity, 92% similarity) [Pubmed|19407374]

    • Additional information

  • It contains a cysteine on the active site. The enzyme is a dimer. [Pubmed|15231785]

    • Expression and Regulation

    Operon

  • ''[SW|polY1]-[protein|search|gndA]'' (according to [http://dbtbs.hgc.jp/COG/prom/yqjHI.html DBTBS])

    • Additional information

  • belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

    • Biological materials

    Mutant

  • GP1514 (''gndA''::''kan''), available in [SW|Jörg Stülke]'s lab

    • Expression vector

  • pGP1777 (for expression, purification in ''E. coli'' with N-terminal Strep-tag, in [SW|pGP172], available in [SW|Jörg Stülke]'s lab)
  • pGP1789 (N-terminal Strep-tag, for [SW|SPINE], purification from ''B. subtilis'', in [SW|pGP1389]) (available in [SW|Jörg Stülke]'s lab)
  • GP1408 (''gndA''-''Strep'' ''(spc)'') & GP1410 (''gndA''-''Strep'' ''(cat)''), purification from ''B. subtilis'', for [SW|SPINE], available in [SW|Jörg Stülke]'s lab

    • two-hybrid system

  • ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW|Jörg Stülke]'s lab

    • FLAG-tag construct

  • GP1403 (spc, based on [SW|pGP1331]), available in [SW|Jörg Stülke]'s lab
  • GP1408 (kan, resistance cassette exchange in GP1403), available in [SW|Jörg Stülke]'s lab

    • Antibody

  • **

    • References

  • 17218307,19407374,15378759,15231785