floT [Tue Dec 13 2016 11:16:35 GMT+0100 (CET)]

membrane-associated scaffold protein, orchestration of physiological processes in lipid microdomains, involved in the control of membrane fluidity, confers (together with YuaF) resistance to cefuroxime

locus: BSU31010

pI: 5.00

mw: 55.00 kDa

function: involved in the control of membrane fluidity

product: membrane-associated scaffold protein

essential: no

synonyms: yuaG, yuaH

Genomic Context

categories

• [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.7|Membrane dynamics]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
• [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.5|Other proteins required for biofilm formation]
• [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.3|Sporulation/ other]
• [category|SW 4|Lifestyles] → [category|SW 4.3|Coping with stress] → [category|SW 4.3.2|Cell envelope stress proteins (controlled by SigM, V, W, X, Y)]
• [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

[SW|Categories] containing this gene/protein

• [SW|biofilm formation], [SW|membrane dynamics], [SW|membrane proteins], [SW|sporulation/ other], [SW|cell envelope stress proteins (controlled by SigM, V, W, X, Y)]

This gene is a member of the following [SW|regulons]

• [SW|SigW regulon]

Gene

Coordinates on the chromosome (coding sequence): 3,180,465 -> 3,181,994

Phenotypes of a mutant

• delayed onset of sporulation, reduced sporulation frequency
• defect in motility [Pubmed|22753055]
• reduced [SW|protein secretion] [Pubmed|23651456]
• a ''[protein|search|floT] [protein|search|floA]'' double mutant does not induce [protein|search|KinC]-dependent biofilm formation upon addition of surfactin [Pubmed|20713508]
• a ''[protein|search|floT] [protein|search|floA]'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency [Pubmed|22753055]
• a ''[protein|search|floT] [protein|search|floA]'' double mutant has a [SW|sporulation] defect, due to the lack of [protein|search|FtsH] [Pubmed|22882210]
• a ''[protein|search|floT]'' mutant displays a defective growth under oxygen-limiting conditions [Pubmed|25909364]

The protein

Catalyzed reaction/ biological activity

• [protein|search|SigW]-dependent expression of ''[protein|search|fabF]'' and the ''[protein|search|yuaF]-[protein|search|floT]-[protein|search|yuaI]'' operon result in reduced membrane fluidity [Pubmed|21542858,22178969]
• recruits [protein|search|YuaF] to focal assemblies [Pubmed|22753055]
• controls protease activity of [protein|search|FtsH] [Pubmed|24222488]

[SW|Localization]

• membrane associated [Pubmed|18763711]
• co-localizes with [protein|search|FloA] and [protein|search|KinC] in discrete foci membrane [Pubmed|20713508]
• forms discrete focal structures in the cytoplasma membrane [Pubmed|22753055]
• 6 foci [protein|search|FloT] of are detected in strain 3610 [Pubmed|25909364]

[SW|Interactions]

• forms homo-oligomers [Pubmed|23651456]
• oligomerization depends on the glutamate-alanine repeats [Pubmed|25909364]
• [protein|search|YuaF]-[protein|search|FloT] [Pubmed|22753055]
• [protein|search|FloT]-[protein|search|FtsH] [Pubmed|26297017,22882210]
• [protein|search|FloT]-[protein|search|FloA] [Pubmed|26297017,23651456]
• [protein|search|FloT]-[protein|search|SecY] [Pubmed|23651456]
• [protein|search|FloT]-[protein|search|FtsX] [Pubmed|23651456]
• [protein|search|FloT]-[protein|search|OppA] [Pubmed|23651456]
• [protein|search|FloT]-[protein|search|SdhA] [Pubmed|23651456]
• [protein|search|FloT]-[protein|search|ResE] [Pubmed|26297017,25909364]
• [protein|search|FloT]-[protein|search|KinC] to stimulate [protein|search|KinC] dimerization and activity [Pubmed|26297017]
• [protein|search|FloT]-[protein|search|KinD] [Pubmed|26297017]

Expression and Regulation

Operon

• ''[protein|search|yuaF]-[protein|search|floT]-[protein|search|yuaI]'' [Pubmed|9987136]

[SW|Sigma factor]

• [protein|search|SigW] [Pubmed|9987136]

Regulation

• expressed upon cell wall stress ([protein|search|SigW]) [Pubmed|9987136]
• repressed by casamino acids [Pubmed|12107147]

Biological materials

Mutant

• JS152 (markerless), available in [SW|Daniel Lopez]'s lab

GFP fusion

• GK38 (168 ''amyE''::P''floT-yfp'' (spc)), available in [SW|Daniel Lopez]'s lab
• JS280 (3610 ''amyE::floT-gfp''(spc)), available in [SW|Daniel Lopez]'s lab
• JS153 (3610 ''lacA::floT''-mEos2 (mls)), available in [SW|Daniel Lopez]'s lab
• JS166 (3610 ''lacA::floT''-PAmCherry (mls)), available in [SW|Daniel Lopez]'s lab

two-hybrid system

• ''B. pertussis'' adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]) for FloT [Pubmed|25909364], available in [SW|Daniel Lopez]'s lab

References

Reviews

• 25652542

Original publications

• 9987136,22753055,12107147,18763711,19383680,20713508,23651456,22178969,23249255,22882210,25635948,25909364,24222488,26297017,27362352