narH

nitrate reductase (beta subunit)

Locus: BSU_37270

Isoelectric point: 5.11

Molecular weight: 55.30 kDa

Protein length: 487 aa

Gene length: 1464 bp

Function: nitrate respiration, nitrogen assimilation

Product: nitrate reductase (beta subunit)

Essential: no

Synonyms

Genomic Context

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Categories containing this gene/protein

• Metabolism → Electron transport and ATP synthesis → Respiration → Anaerobic respiration
• Groups of genes → Membrane proteins

Gene

Coordinates: 3,824,806 3,826,269

The protein

Catalyzed reaction/ biological activity

• quinol + nitrate --> quinone + H₂O + nitrite (according to UniProt)

Domains

• 3 4Fe-4S ferredoxin-type domains (aa 7-36, aa 172-203, aa 205-234) (according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 1Q16 (the NarG-NarH-NarI complex of E. coli, 59% identity) PubMed

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: narG-narH-narJ-narI
  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

  Regulatory mechanism

  • Fnr: activation, PubMed, in Fnr regulon

  Regulation

  • expressed under anaerobic conditions (Fnr) PubMed

Biological materials

Mutant

• BKE37270 (narH::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTTCATGACCATACCGATTT, downstream forward: _UP4_TAACTTTTGATAAATGAGGT
• BKK37270 (narH::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTTCATGACCATACCGATTT, downstream forward: _UP4_TAACTTTTGATAAATGAGGT

References

Reviews

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Original publications

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