rocD [Mon May 16 2016 17:50:37 GMT+0200 (CEST)]

ornithine transaminase

locus: BSU40340

pI: 4.00

mw: 43.00 kDa

function: arginine, ornithine and citrulline utilization

product: ornithine transaminase

essential: no

ec: 2.6.1.13

synonyms

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.3|Amino acid/ nitrogen metabolism] → [category|SW 2.3.2|Utilization of amino acids] → [category|SW 2.3.2.4|Utilization of arginine/ ornithine]

[SW|Categories] containing this gene/protein

• [SW|utilization of amino acids]

This gene is a member of the following [SW|regulons]

• [SW|AhrC regulon], [SW|CodY regulon], [SW|RocR regulon], [SW|SigL regulon], [SW|Spo0A regulon]

Gene

Coordinates on the chromosome (coding sequence): 4,144,301 -> 4,145,506

The protein

Catalyzed reaction/ biological activity

• L-ornithine a 2-oxo acid = L-glutamate 5-semialdehyde an L-amino acid (according to Swiss-Prot)

Protein family

• OAT subfamily (according to Swiss-Prot)

Structure

• [PDB|3RUY] (from ''B. anthracis'')

Expression and Regulation

Operon

• ''[protein|search|rocD]-[protein|search|rocE]-[protein|search|rocF]'' [Pubmed|7540694]

[SW|Sigma factor]

• [protein|search|SigL] [Pubmed|7540694]

Regulation

• induced by arginine ([protein|search|RocR], [protein|search|AhrC]) [Pubmed|7540694]
• repressed under conditions that trigger sporulation ([SW|Spo0A]) [Pubmed|14651647]
• repressed during growth in the presence of branched chain amino acids ([protein|search|CodY]) [Pubmed|12618455]

Regulatory mechanism

• [protein|search|RocR], [protein|search|AhrC]: transcription activation [Pubmed|7540694]
• [SW|Spo0A]: transcription repression [Pubmed|14651647]
• [protein|search|CodY]: transcription repression [Pubmed|12618455]

Biological materials

Mutant

• GP656, aphA3, available in [SW|Jörg Stülke]'s lab

References

• 12618455,23869754,14651647,12618455,7540694