sodA

superoxide dismutase, general stress protein, important for survival of ethanol and paraquat stresses and at low temperatures

Locus: BSU_25020

Isoelectric point: 5.20

Molecular weight: 22.43 kDa

Protein length: 202 aa

Gene length: 609 bp

Function: detoxification of oxygen radicals

Product: superoxide dismutase

Essential: no

E.C.: 1.15.1.1

Synonyms: yqgD

Genomic Context

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Categories containing this gene/protein

• Lifestyles → Coping with stress → General stress proteins (controlled by SigB)
• Lifestyles → Coping with stress → Resistance against oxidative and electrophile stress
• Groups of genes → Phosphoproteins → Phosphorylation on a Thr residue

Gene

Coordinates: 2,585,434 2,586,042

The protein

Catalyzed reaction/ biological activity

• 2 H⁺ + 2 superoxide --> H₂O₂ + O₂ (according to UniProt)

Protein family

• iron/manganese superoxide dismutase family (with SodF, according to UniProt)

Paralogous protein(s)

• SodF

Modification

• phosphorylation on Thr-34 AND Thr-70 PubMed

Cofactors

• manganese

Structure

• 2RCV PubMed

Localization

• cytoplasm PubMed

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: yqgC-sodA
  Description: PubMed

• Genes: sodA
  Description: PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in SigB regulon

  Regulation

  • induced by stress (SigB) PubMed
  • constitutive expression PubMed

Biological materials

Mutant

• GP3165 sodA::spec trpC2 available in Jörg Stülke's lab
• MGNA-C451 (yqgD::erm), available at the NBRP B. subtilis, Japan
• BKE25020 (sodA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATAATTCCTCCTTAGT, downstream forward: _UP4_TAATGGCACAAACAAGGTCC
• BKK25020 (sodA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATGATAATTCCTCCTTAGT, downstream forward: _UP4_TAATGGCACAAACAAGGTCC

References

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