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ptkA [2019-02-19 19:22:30]
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ptkA [2019-02-19 19:22:30]

protein tyrosine kinase
Locus
BSU36250
Isoelectric point
9.63
Molecular weight
25.64 kDa
Protein length
237 aa Sequence Blast
Gene length
714 bp Sequence Blast
Function
protein phosphorylation
Product
protein tyrosine kinase
Essential
no
Synonyms
ywqD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      
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Categories containing this gene/protein

  • Information processing → Protein synthesis, modification and degradation → Protein modification → Protein kinases
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation
  • Lifestyles → Exponential and early post-exponential lifestyles → Biofilm formation → Regulation
  • Groups of genes → Phosphoproteins → Phosphorylation on a Tyr residue

    • Gene

    Coordinates
    3,731,822 3,732,535

    Phenotypes of a mutant

  • Accumulation of extra chromosome equivalents PubMed
  • Defect in biofilm formation, this involves the kinase activity, but the target protein is unknown PubMed

    • The protein

    Catalyzed reaction/ biological activity

  • ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate (according to Swiss-Prot), autophosphorylation, phosphorylation of Ugd, TuaD, SsbB, SsbB

    • Protein family

  • BY-kinase, see the Bacterial Protein Tyrosine Kinase Database)

    • Paralogous protein(s)

  • EpsB

    • Domains

  • single BY-kinase domain

    • Modification

  • autophosphorylation at residues Y225, Y227 and Y228 (primary site) PubMed, dephosphorylated by PtpZ PubMed

    • Cofactors

  • ATP

    • Effectors of protein activity

  • TkmA - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation PubMed
  • the mutually exclusive interactions of PtsA with the modulator proteins TkmA, SalA, and probably MinD direct the kinase to different substrates PubMed

    • Structure

  • 2VED (CapB, the homolog in Staphylococcus aureus)

    • Expression and Regulation

    Operons

    Genes
    tkmA-ptkA-ptpZ-ugd
    Description
    PubMed

    Sigma factors

  • SigA: sigma factor, PubMed, in SigA regulon

    • Regulatory mechanism

  • Spo0A: activation, PubMed, in Spo0A regulon
  • DegU: activation, DegU-P, PubMed, in DegU regulon
  • AbrB: repression, PubMed, in AbrB regulon
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    Biological materials

    Mutant

  • MGNA-A078 (ywqD::erm), available at the NBRP B. subtilis, Japan
  • KO strain created with pMUTIN-2, available from Ivan Mijakovic
  • GP1520 (spc), available in Jrg Stlke's lab
  • GP1544 (ermC), available in Jrg Stlke's lab
  • GP1587 (cat) , available in Jrg Stlke's lab
  • GP1521 epsB (aphA3) ptkA (spc) double mutant available in Jrg Stlke's lab
  • GP1529 tkmA-ptkA::spc available in Jrg Stlke's lab
  • GP1610 (ptkA-ptpZ, spc), available in Jrg Stlke's lab
  • BKE36250 (ptkA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTGCATCCGCGAGCCTCTGT, downstream forward: _UP4_TAAATAACGTGCATCGTGCC
  • BKK36250 (ptkA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CTGCATCCGCGAGCCTCTGT, downstream forward: _UP4_TAAATAACGTGCATCGTGCC

    • Expression vectors

  • pQE-30, N-terminally 6xHis-tagged, available from Ivan Mijakovic

    • LacZ fusion

  • in a KO strain created with pMUTIN-2, available from Ivan Mijakovic

    • GFP fusion

  • CFP-fusion, available from Ivan Mijakovic

    • Two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jrg Stlke's lab

    • Labs working on this gene/protein

  • Ivan Mijakovic, Thiverval-Grignon, France

    • References

    Reviews

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    Original publications

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