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ptkA [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]
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ptkA [Tue Dec 13 2016 11:16:36 GMT+0100 (CET)]

protein tyrosine kinase
locus
BSU36250
pI
9.00
mw
25.00 kDa
function
protein phosphorylation
product
protein tyrosine kinase
essential
no
synonyms
ywqD
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 3|Information processing] → [category|SW 3.3|Protein synthesis, modification and degradation] → [category|SW 3.3.4|Protein modification] → [category|SW 3.3.4.2|Protein kinases]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation]
  • [category|SW 4|Lifestyles] → [category|SW 4.1|Exponential and early post-exponential lifestyles] → [category|SW 4.1.2|Biofilm formation] → [category|SW 4.1.2.4|Regulation]
  • [category|SW 6|Groups of genes] → [category|SW 6.4|Phosphoproteins] → [category|SW 6.4.7|Phosphorylation on a Tyr residue]

    • [SW|Categories] containing this gene/protein

  • [SW|biofilm formation], [SW|protein modification], [SW|membrane proteins]

    • This gene is a member of the following [SW|regulons]

  • [SW|AbrB regulon], [SW|DegU regulon], [SW|Spo0A regulon]

    • Gene

    Coordinates on the chromosome (coding sequence)
    3,731,822 -> 3,732,535

    Phenotypes of a mutant

  • Accumulation of extra chromosome equivalents [Pubmed|17367396]
  • Defect in [SW|biofilm formation], this involves the kinase activity, but the target protein is unknown [Pubmed|20815827]

    • The protein

    Catalyzed reaction/ biological activity

  • ATP a [protein]-L-tyrosine = ADP a [protein]-L-tyrosine phosphate (according to Swiss-Prot), autophosphorylation, phosphorylation of [protein|search|Ugd], [protein|search|TuaD], [protein|search|Ssb], [protein|search|SsbB]

    • Protein family

  • BY-kinase, see the [http://bykdb.ibcp.fr/BYKdb/BYKdbHelp Bacterial Protein Tyrosine Kinase Database])

    • Paralogous protein(s)

  • [protein|search|EpsB]

    • [SW|Domains]

  • single BY-kinase domain

    • Modification

  • autophosphorylation at residues Y225, Y227 and Y228 (primary site) [Pubmed|20509597], dephosphorylated by [protein|search|PtpZ] [Pubmed|15866923]

    • [SW|Cofactors]

  • ATP

    • Effectors of protein activity

  • [protein|search|TkmA] - transmembrane modulator, activates PtkA autophosphorylation and substrate phosphorylation [Pubmed|12970183]
  • the mutually exclusive interactions of PtsA with the modulator proteins [SW|TkmA], [SW|SalA], and probably [SW|MinD] direct the kinase to different substrates [Pubmed|27725816]

    • Structure

  • [PDB|2VED] (CapB, the homolog in ''Staphylococcus aureus'')

    • [SW|Interactions]

  • [protein|search|PtkA]-[protein|search|TkmA] [Pubmed|12970183]
  • [protein|search|FatR]-[protein|search|PtkA] [Pubmed|23939619]
  • [SW|SalA]-[SW|PtkA], for phosphorylation of [SW|SalA] [Pubmed|26094643]
  • [SW|DnaK]-[SW|PtkA] (for phosphorylation of [SW|DnaK]) [Pubmed|27148221]

    • Expression and Regulation

    Operon

  • ''[protein|search|tkmA]-[protein|search|ptkA]-[protein|search|ptpZ]-[protein|search|ugd]'' [Pubmed|20815827,12970183]

    • [SW|Sigma factor]

  • [protein|search|SigA] [Pubmed|20815827]

    • Regulatory mechanism

  • [SW|Spo0A]: transcription activation [Pubmed|26283769]
  • [protein|search|DegU]-P: transcription activation [Pubmed|26283769]
  • [protein|search|AbrB]: transcription repression [Pubmed|20817675]

    • Biological materials

    Mutant

  • KO strain created with pMUTIN-2, available from [SW|Ivan Mijakovic]
  • GP1520 (spc), available in [SW| Jörg Stülke]'s lab
  • GP1544 (ermC), available in [SW| Jörg Stülke]'s lab
  • GP1587 (cat) , available in [SW| Jörg Stülke]'s lab
  • GP1521 ''[protein|search|epsB]'' (aphA3) ''[protein|search|ptkA]'' (spc) double mutant available in [SW| Jörg Stülke]'s lab
  • GP1529 ''[protein|search|tkmA]-[protein|search|ptkA]''::spc available in [SW| Jörg Stülke]'s lab
  • GP1610 (''[protein|search|ptkA]-[protein|search|ptpZ]'', spc), available in [SW| Jörg Stülke]'s lab

    • Expression vector

  • pQE-30, N-terminally 6xHis-tagged, available from [SW|Ivan Mijakovic]

    • lacZ fusion

  • in a KO strain created with pMUTIN-2, available from [SW|Ivan Mijakovic]

    • GFP fusion

  • CFP-fusion, available from [SW|Ivan Mijakovic]

    • two-hybrid system

  • B. pertussis adenylate cyclase-based bacterial two hybrid system ([SW|BACTH]), available in [SW| Jörg Stülke]'s lab

    • Labs working on this gene/protein

  • [SW|Ivan Mijakovic], Thiverval-Grignon, France

    • References

    Reviews

  • 20497498,24554699,25540643,25667587,26286503

    • Original publications

  • 12970183,15741737,15866923,17367396,19258708,18547145,20497499,20509597,20815827,20817675,23939619,24493247,24728941,25278935,25374563,26283769,26094643,27148221,27725816