fabI [2018-02-10T14:15:22.000Z]

enoyl-acyl carrier protein reductase

locus: BSU11720

pI: 5.61

mw: 27.72 kDa

protein length: 258 aa

gene length: 774 bp

function: fatty acid biosynthesis

product: enoyl-acyl carrier protein reductase

essential: no

ec: 1.3.1.9

synonyms: yjbW

Genomic Context

categories

• [category|SW 2|Metabolism] → [category|SW 2.4|Lipid metabolism] → [category|SW 2.4.2|Biosynthesis of lipids] → [category|SW 2.4.2.1|Biosynthesis of fatty acids]

Gene

Coordinates: 1,247,788 → 1,248,564

The protein

Catalyzed reaction/ biological activity

• Acyl-[acyl-carrier-protein] NAD = trans-2,3-dehydroacyl-[acyl-carrier-protein] NADH (according to Swiss-Prot)

Protein family

• FabI subfamily (according to Swiss-Prot)

Paralogous protein(s)

• [protein|5964B6E817260DA7937796DDFA753A665A04D650|FabL], one of the two proteins has to be present for viability [Pubmed|17114254]

Effectors of protein activity

• inhibited by triclosan [Pubmed|11325930]

Structure

• [PDB|3OIF] (complex with NAD and inhibitor triclosan) [Pubmed|21185310]

additional information

• belongs to the 100 [SW|most abundant proteins] [PubMed|15378759]

Expression and Regulation

Operons

• genes: [gene|60A519E4C1485B9F5BF36E83EDE884B396857CB4|fabI]
  description: [Pubmed|12737802]

  sigma factors

  • [protein|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI]: sigma factor, [pubmed|29914988], in [regulon|3DEAC421B4B173C6BBC700E57790751B7AFDF319|SigI regulon]

  regulatory mechanism

  • [protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]: repression, [Pubmed|12737802], in [regulon|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR regulon]

  regulation

  • expressed when the cells experience a lack of malonyl-CoA ([protein|FCDE900167AE36377979E0CF7BC33C7F351B95D3|FapR]) [Pubmed|12737802]
  • expression of the operon is strongly reduced during [SW|biofilm formation] [pubmed|31113899]

Biological materials

Mutant

• MGNA-B168 (yjbW::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1167 NBRP B. subtilis, Japan]
• BKE11720 (Δ[gene|60A519E4C1485B9F5BF36E83EDE884B396857CB4|fabI]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE11720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTATAAAGCCTCCTA, downstream forward: _UP4_TAAGCAGGATCTATATCAAG
• BKK11720 (Δ[gene|60A519E4C1485B9F5BF36E83EDE884B396857CB4|fabI]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK11720 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATATGTATAAAGCCTCCTA, downstream forward: _UP4_TAAGCAGGATCTATATCAAG

References

Reviews

• 15952903,17919287

Original Publications

• 12737802,17114254,8953047,11007778,21185310,21383089,15378759