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rodZ [2020-04-05 12:59:45]
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rodZ [2020-04-05 12:59:45]

required for proper septum positioning during vegetative growth ([SW|cell shape] determination), part of the [protein|search|Rod complex] for lateral [SW|cell wall synthesis] and control of cell diameter
locus
BSU_16910
pI
4.68
mw
23.48 kDa
protein length
288 aa Sequence Blast
gene length
867 bp Sequence Blast
function
septum positioning during vegetative growth
product
morphogenic protein
essential
yes
synonyms
ymfM
Outlinks
KEGGBsubCycSubtiListUniProtExpression Browser

Genomic Context

      

categories

  • [category|SW 1|Cellular processes] → [category|SW 1.1|Cell envelope and cell division] → [category|SW 1.1.2|Cell shape]
  • [category|SW 4|Lifestyles] → [category|SW 4.2|Sporulation] → [category|SW 4.2.3|Sporulation/ other]
  • [category|SW 6|Groups of genes] → [category|SW 6.2|Membrane proteins]

    • Gene

    Coordinates
    1,761,707 1,762,573

    Phenotypes of a mutant

  • essential [Pubmed|19164570,23879732]
  • depletion results in the formation of shorter and rounder cells [Pubmed|23879732]
  • depletion results in disturbed formation of asymmetric septa and perturbed [protein|CFF318EE6CACDEE763FBF96A2ABB46E944679AAA|SigF] activation [Pubmed|27415800]
  • hypersensitivity to sorbic acid stress [Pubmed|27818647]
  • small, round, DNA-containing cells [pubmed|29403445]

    • The protein

    [SW|Domains]

  • N-terminal cytoplasmic domain (RodZn), a transmembrane domain, and C-terminal extra-cytoplasmic domain (RodZc) [Pubmed|25503291]

    • [SW|Localization]

  • membrane protein (according to UniProt)

    • Additional information

  • overexpression results in growth inhibition in minimal medium (in the absence of Mg2+ ) [Pubmed|19164570]
  • RodZ is destabilized in a ''[gene|A4C8719E06F774A6EB4D79757CC79CF89E453A54|mreB]'' mutant [Pubmed|23879732]

    • Expression and Regulation

    Operons

    genes
    [gene|725094DC9353EBB5FD603AB389607D8C13DF5F51|ymfK/1]-[gene|9394EA0675DFFCB5F0ECD494C104A66CE13A8EF6|ymfK/2]-[gene|6071A84EF190C820DB8985C08ABC74F744B793DB|rodZ]
    description
    [Pubmed|22383849]
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    Biological materials

    Mutant

  • MGNA-B120 (ymfM::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1119 NBRP B. subtilis, Japan]
  • BKE16910 ([gene|6071A84EF190C820DB8985C08ABC74F744B793DB|rodZ]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE16910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTTTTCCTCTCTGG, downstream forward: _UP4_TAATTACCAGATGACTTTTC
  • BKK16910 ([gene|6071A84EF190C820DB8985C08ABC74F744B793DB|rodZ]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK16910 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATTGCTTTTTCCTCTCTGG, downstream forward: _UP4_TAATTACCAGATGACTTTTC

    • labs

  • [SW|Adriano Henriques], Lisbon, Portugal [http://www.itqb.unl.pt/research/biology/microbial-development homepage]

    • References

  • 19164570,21636744,21636745,23879732,25503291,27415800,27818647,29403445,30651563