function
degradation of polygalacturonic acid
Genomic Context
categories
[category|SW 2|Metabolism] → [category|SW 2.2|Carbon metabolism] → [category|SW 2.2.2|Utilization of specific carbon sources] → [category|SW 2.2.2.27|Utilization of other polymeric carbohydrates][category|SW 6|Groups of genes] → [category|SW 6.12|Secreted proteins][SW|Categories] containing this gene/protein
[SW|utilization of specific carbon sources]This gene is a member of the following [SW|regulons]
[SW|CcpA regulon], [SW|ComA regulon], [SW|TnrA regulon]Gene
Coordinates on the chromosome (coding sequence)
827,993 -> 829,255
The protein
Catalyzed reaction/ biological activity
Eliminative cleavage of (1->4)-alpha-D-galacturonan to give oligosaccharides with 4-deoxy-alpha-D-galact-4-enuronosyl groups at their non-reducing ends (according to Swiss-Prot)Protein family
polysaccharide lyase 1 family (according to Swiss-Prot)Structure
[PDB|2O1D] (bound to trisaccharide), [PDB|1BN8][SW|Localization]
extracellular (signal peptide), major constituent of the secretome [Pubmed|18957862]Expression and Regulation
Operon
''pel'' [Pubmed|12823818]Regulation
repressed by glucose (3.8-fold) ([protein|search|CcpA]) [Pubmed|12850135]repressed in the absence of good nitrogen sources (glutamine or ammonium) ([protein|search|TnrA]) [Pubmed|12823818]expressed at high cell density ([protein|search|ComA]) [Pubmed|16091051]Regulatory mechanism
[protein|search|ComA]: transcription activation ([protein|search|ComA]) [Pubmed|16091051][protein|search|CcpA]: transcription repression[protein|search|TnrA]: transcription repression [Pubmed|12823818]Biological materials
Mutant
MGNA-C248 (pel::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/2246 NBRP B. subtilis, Japan]References
Reviews
Original publications
8262178,16091051,12823818,18957862,12850135,25755103,7634076,26582911,27447541 
